PMID- 8574897
OWN - NLM
STAT- MEDLINE
DCOM- 19960312
LR  - 20191101
VI  - 12
IP  - 2
DP  - 1995 Oct
TI  - Characterization of a human chromosome 22 enriched bacterial artificial 
      chromosome sublibrary.
PG  - 73-9
AB  - Selection of chromosomal sublibraries from total human genomic libraries is 
      critical for chromosome-based physical mapping approaches. We have previously 
      reported a method of screening total human genomic library using flow sorted 
      chromosomal DNA as a hybridization probe and selection of a human chromosome 
      22-enriched sublibrary from a total human bacterial artificial chromosome (BAC) 
      library (Nucleic Acids Res 1995; 23: 1838-39). We describe here further details 
      of the method of construction as well as characterization of the chromosome 
      22-enriched sublibrary thus constructed. Nearly 40% of the BAC clones that have 
      been mapped by fluorescence in situ hybridization (FISH) analysis were localized 
      to chromosome 22. By screening the sublibrary using chromosome 22-specific 
      hybridization probes, we estimated that the sublibrary represents at least 2.5 x 
      coverage of chromosome 22. This is in good agreement with the results from FISH 
      mapping experiments. FISH map data also indicate that chromosome 22-specific BACs 
      in the sublibrary represent all the subregions of chromosome 22.
FAU - Kim, U J
AU  - Kim UJ
AD  - Division of Biology and Beckmann Institute, California Institute of Technology, 
      Pasadena 91125, USA.
FAU - Shizuya, H
AU  - Shizuya H
FAU - Chen, X N
AU  - Chen XN
FAU - Deaven, L
AU  - Deaven L
FAU - Speicher, S
AU  - Speicher S
FAU - Solomon, J
AU  - Solomon J
FAU - Korenberg, J
AU  - Korenberg J
FAU - Simon, M I
AU  - Simon MI
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Genet Anal
JT  - Genetic analysis : biomolecular engineering
JID - 9509403
SB  - IM
MH  - Animals
MH  - *Chromosomes, Bacterial
MH  - *Chromosomes, Human, Pair 22
MH  - Flow Cytometry
MH  - *Gene Library
MH  - *Genome, Human
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1016/1050-3862(95)00121-2 [doi]
PST - ppublish
SO  - Genet Anal. 1995 Oct;12(2):73-9. doi: 10.1016/1050-3862(95)00121-2.