PMID- 8575280
OWN - NLM
STAT- MEDLINE
DCOM- 19960312
LR  - 20190909
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 13
IP  - 3
DP  - 1995 Oct
TI  - Cytology, flow cytometry, image analysis, and interphase cytogenetics by 
      fluorescence in situ hybridization in the diagnosis of transitional cell 
      carcinoma in bladder washes: a comparative study.
PG  - 214-23; discussion 224
AB  - The diagnosis of transitional cell carcinoma (TCC) in bladder washes is a 
      diagnostic challenge to cytology. This study assessed the role of flow cytometry 
      (FCM), image analysis (IA), and interphase cytogenetics by fluorescence in situ 
      hybridization (FISH) as adjuncts in the cytodiagnosis of TCC in bladder washes. 
      Forty separate samples of bladder washes were prospectively evaluated by 
      conventional cytology (CY), FCM, IA, and FISH, and the results were compared with 
      the subsequent surgical biopsy specimens which revealed 26 TCC (3 GR I, 6 GR II, 
      17 GR III) and 14 benign lesions. Using histology as the "gold standard" and 
      following the previously published criteria for detection of TCC by CY, FCM, IA, 
      and FISH, the concordance rates between histology and CY, FCM, IA, and FISH were 
      75, 74, 89, and 83%, respectively. CY, FCM, IA, FISH, and histology were 
      concordant in 54% of the cases. The sensitivity of CY, FCM, IA, and FISH were 61, 
      72, 91, and 73%, respectively, while the specificity were 100, 80, 83, and 100%, 
      respectively. The combined sensitivity of all the parameters was 96%. 
      Interestingly, the false positive cases by FCM and IA showed cystitis. We 
      conclude that IA has the highest sensitivity in detecting TCC in bladder washes 
      followed by FISH, FCM, and CY, while CY and FISH have the highest specificity. 
      This study indicates that FCM, IA, and FISH are useful adjuncts to cytology in 
      the diagnosis of TCC in bladder washes. The finding of DNA-aneuploidy in cystitis 
      warrants further investigation.
FAU - Cajulis, R S
AU  - Cajulis RS
AD  - Section of Cytology, Northwestern University Medical School, Chicago, IL 
      60611-3008, USA.
FAU - Haines, G K 3rd
AU  - Haines GK 3rd
FAU - Frias-Hidvegi, D
AU  - Frias-Hidvegi D
FAU - McVary, K
AU  - McVary K
FAU - Bacus, J W
AU  - Bacus JW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
SB  - IM
MH  - Carcinoma, Transitional Cell/diagnosis/genetics/*pathology
MH  - *Flow Cytometry
MH  - Humans
MH  - *Image Processing, Computer-Assisted
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase/*genetics
MH  - Urinary Bladder Neoplasms/diagnosis/genetics/*pathology
EDAT- 1995/10/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1002/dc.2840130307 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 1995 Oct;13(3):214-23; discussion 224. doi: 
      10.1002/dc.2840130307.