PMID- 8575759
OWN - NLM
STAT- MEDLINE
DCOM- 19960314
LR  - 20190905
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 29
IP  - 3
DP  - 1995 Oct 10
TI  - A 6-Mb yeast artificial chromosome contig and long-range physical map 
      encompassing the region on chromosome 12q15 frequently rearranged in a variety of 
      benign solid tumors.
PG  - 665-78
AB  - Cytogenetic analysis of a variety of benign solid tumors, among which uterine 
      leiomyoma, lipoma, pleomorphic salivary gland adenoma, and pulmonary chondroid 
      hamartoma, has indicated that these tumors often display chromosome breakpoints 
      in region q13-q15 of chromosome 12. In previous studies, we have reported that 
      these breakpoints map between locus D12S8 and the CHOP gene, the latter of which 
      has been shown to be consistently rearranged in myxoid liposarcomas with 
      t(12;16)(q13;p11). Here, we report directional chromosome walking studies 
      starting from D12S8 and resulting in the construction of a YAC contig of about 6 
      Mb. This YAC contig, whose orientation on chromosome 12 was determined by 
      double-color fluorescence in situ hybridization (FISH) analysis, has at least 
      double coverage and consists of 75 overlapping YAC clones, all isolated from CEPH 
      YAC libraries. Their insert sizes were estimated by contour-clamped homogeneous 
      electric field (CHEF) gel electrophoresis. Chromosomal localization and chimerism 
      of the YACs were investigated by FISH analysis. Chimerism of YAC clones was 
      independently determined by restriction mapping. On the basis of YAC end-derived 
      DNA markers and sequence-tagged sites (STSs), with an average spacing of 
      approximately 70 kb, as well as restriction enzyme analysis, a long-range 
      physical map was established for the 6-Mb DNA region of chromosome 12 covered by 
      the YAC contig. Within the YAC contig, the relative positions of various known 
      genes, an expressed sequence-tagged site, and a number of CEPH/Genethon 
      polymorphic markers were determined. The latter data allow full integration of 
      our mapping data with those obtained by CEPH/Genethon as well as those reported 
      at the Second International Workshop on Human Chromosome 12 Mapping. Finally, 
      this YAC contig constitutes the basis for the contstruction of a transcriptional 
      map of this region and is likely to facilitate identification of genes involved 
      in the formation of various benign solid tumor types.
FAU - Schoenmakers, E F
AU  - Schoenmakers EF
AD  - Laboratory for Molecular Oncology, Center for Human Genetics, University of 
      Leuven, Belgium.
FAU - Geurts, J M
AU  - Geurts JM
FAU - Kools, P F
AU  - Kools PF
FAU - Mols, R
AU  - Mols R
FAU - Huysmans, C
AU  - Huysmans C
FAU - Bullerdiek, J
AU  - Bullerdiek J
FAU - Van den Berghe, H
AU  - Van den Berghe H
FAU - Van de Ven, W J
AU  - Van de Ven WJ
LA  - eng
SI  - GENBANK/U28792
SI  - GENBANK/U28793
SI  - GENBANK/U28794
SI  - GENBANK/U28994
SI  - GENBANK/U28995
SI  - GENBANK/U28996
SI  - GENBANK/U28997
SI  - GENBANK/U28998
SI  - GENBANK/U28999
SI  - GENBANK/U29000
SI  - GENBANK/U29001
SI  - GENBANK/U29002
SI  - GENBANK/U29003
SI  - GENBANK/U29004
SI  - GENBANK/U29005
SI  - GENBANK/U29006
SI  - GENBANK/U29007
SI  - GENBANK/U29008
SI  - GENBANK/U29009
SI  - GENBANK/U29010
SI  - GENBANK/U29011
SI  - GENBANK/U29012
SI  - GENBANK/U29013
SI  - GENBANK/U29014
SI  - GENBANK/U29015
SI  - GENBANK/U29016
SI  - GENBANK/U29017
SI  - GENBANK/U29018
SI  - GENBANK/U29019
SI  - GENBANK/U29020
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DDIT3 protein, human)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Genetic Markers)
RN  - 0 (Transcription Factors)
RN  - 147336-12-7 (Transcription Factor CHOP)
SB  - IM
MH  - Adenoma/genetics
MH  - Base Sequence
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - Chimera
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 16
MH  - DNA Primers
MH  - DNA-Binding Proteins/genetics
MH  - Female
MH  - Gene Library
MH  - Genetic Markers
MH  - Hamartoma/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leiomyoma/genetics
MH  - Lipoma/genetics
MH  - Lung/abnormalities
MH  - Molecular Sequence Data
MH  - Neoplasms/*genetics
MH  - Polymerase Chain Reaction
MH  - Salivary Gland Neoplasms/genetics
MH  - Transcription Factor CHOP
MH  - Transcription Factors/genetics
MH  - Translocation, Genetic
MH  - Uterine Neoplasms/genetics
EDAT- 1995/10/10 00:00
MHDA- 1995/10/10 00:01
CRDT- 1995/10/10 00:00
PHST- 1995/10/10 00:00 [pubmed]
PHST- 1995/10/10 00:01 [medline]
PHST- 1995/10/10 00:00 [entrez]
AID - S0888754385799521 [pii]
AID - 10.1006/geno.1995.9952 [doi]
PST - ppublish
SO  - Genomics. 1995 Oct 10;29(3):665-78. doi: 10.1006/geno.1995.9952.