PMID- 8575971
OWN - NLM
STAT- MEDLINE
DCOM- 19960314
LR  - 20100324
IS  - 0003-1488 (Print)
IS  - 0003-1488 (Linking)
VI  - 208
IP  - 3
DP  - 1996 Feb 1
TI  - Stability of porcine reproductive and respiratory syndrome virus in the presence 
      of fomites commonly found on farms.
PG  - 390-2
AB  - OBJECTIVE: To determine the survival of porcine reproductive and respiratory 
      syndrome virus (PRRSV) on nonliving substances (fomites) at 25 to 27 C. DESIGN: 
      Prospective controlled study. SAMPLE POPULATION: 3 solid, 6 porous, and 7 liquid 
      fomites. PROCEDURE: The fomites were contaminated with known concentrations of 
      PRRSV. Samples for virus isolation were obtained on day 0 through day 11, assayed 
      in cell cultures, and stained with fluorescent antibody conjugate. RESULTS: The 
      virus was recovered only on day-0 samples of alfalfa, wood shavings, straw, 
      plastic, boot rubber, and stainless steel. Virus was isolated from city water 
      through day 11, from well water through day 9, and from 2 buffer solutions for 4 
      and 6 days. The virus was isolated only on day 0 from swine saliva, urine, and 
      fecal slurry. CLINICAL IMPLICATIONS: Results indicated that PRRSV is a fairly 
      labile virus, but because of its duration of viability in water, contamination of 
      drinking water and lagoons by PRRSV-shedding swine would serve as sources of 
      virus to infect susceptible swine.
FAU - Pirtle, E C
AU  - Pirtle EC
AD  - Department of Microbiology, Immunology, and Preventive Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames 50011, USA.
FAU - Beran, G W
AU  - Beran GW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Am Vet Med Assoc
JT  - Journal of the American Veterinary Medical Association
JID - 7503067
RN  - 0 (Plastics)
RN  - 12597-68-1 (Stainless Steel)
RN  - 9006-04-6 (Rubber)
SB  - IM
MH  - Animal Feed/virology
MH  - Animals
MH  - Arterivirus/isolation & purification/*physiology
MH  - Arterivirus Infections/veterinary/virology
MH  - *Environmental Microbiology
MH  - Feces/virology
MH  - Medicago sativa/virology
MH  - Plastics
MH  - Poaceae/virology
MH  - Rubber
MH  - Saliva/virology
MH  - Stainless Steel
MH  - Swine
MH  - Swine Diseases/virology
MH  - Urine/virology
MH  - Water Microbiology
MH  - Water Supply
MH  - Wood
MH  - Zea mays/virology
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
PST - ppublish
SO  - J Am Vet Med Assoc. 1996 Feb 1;208(3):390-2.