PMID- 8576949
OWN - NLM
STAT- MEDLINE
DCOM- 19960313
LR  - 20190830
IS  - 0022-2828 (Print)
IS  - 0022-2828 (Linking)
VI  - 27
IP  - 10
DP  - 1995 Oct
TI  - Angiotensin II stimulates the autocrine production of transforming growth 
      factor-beta 1 in adult rat cardiac fibroblasts.
PG  - 2347-57
AB  - Angiotensin II (Ang II) has been implicated in the development of cardiac 
      hypertrophy and myocardial fibrosis. While recent in vivo and in vitro studies 
      performed in cultured cardiac myocytes and fibroblasts support this role for Ang 
      II, the mechanisms of Ang II action at the cellular level remain unclear. In the 
      present study, we postulated that Ang II action in adult cardiac fibroblasts may 
      stimulate the autocrine production and release of transforming growth factor-beta 
      1 (TGF-beta 1), a known regulator of cardiac fibroblast and myocyte function. We 
      examined the ability of Ang II to regulate the gene expression, biological 
      activity, and protein production of TGF-beta 1 in cultured adult rat cardiac 
      fibroblasts. Treatment of fibroblast cultures with Ang II (10(-9) M) induced a 
      two-fold increase in TGF-beta 1 mRNA levels within 4 h that was sustained through 
      24 h (P < 0.01). TGF-beta 1-like activity in Ang II-treated cultures was 
      significantly increased compared with control as measured by bioassay (P < 
      0.001). Specificity for TGF-beta 1-like activity was confirmed through its 
      neutralization with a TGF-beta 1 specific antibody (100 micrograms/ml). Total 
      concentration of TGF-beta 1 (latent plus active forms) in conditioned media from 
      Ang II-treated cardiac fibroblasts was also found to be greater than control (P < 
      0.01). These findings suggest that the effects of Ang II in the adult myocardium 
      may be mediated in part by autocrine/paracrine mechanisms, including the 
      production and release of TGF-beta 1 by cardiac fibroblasts.
FAU - Lee, A A
AU  - Lee AA
AD  - Department of Bioengineering, University of California, San Diego 92103, USA.
FAU - Dillmann, W H
AU  - Dillmann WH
FAU - McCulloch, A D
AU  - McCulloch AD
FAU - Villarreal, F J
AU  - Villarreal FJ
LA  - eng
GR  - HL-43617/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Mol Cell Cardiol
JT  - Journal of molecular and cellular cardiology
JID - 0262322
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Angiotensin II/*pharmacology
MH  - Animals
MH  - Cardiomegaly/metabolism
MH  - Culture Media, Conditioned/chemistry
MH  - Endomyocardial Fibrosis/metabolism
MH  - Fibroblasts/*drug effects/metabolism
MH  - Gene Expression Regulation/*drug effects
MH  - Male
MH  - Myocardium/*cytology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stimulation, Chemical
MH  - Transforming Growth Factor beta/*biosynthesis/genetics/metabolism
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - S0022-2828(95)91983-X [pii]
AID - 10.1016/s0022-2828(95)91983-x [doi]
PST - ppublish
SO  - J Mol Cell Cardiol. 1995 Oct;27(10):2347-57. doi: 10.1016/s0022-2828(95)91983-x.