PMID- 8578170
OWN - NLM
STAT- MEDLINE
DCOM- 19960311
LR  - 20190814
IS  - 0036-5521 (Print)
IS  - 0036-5521 (Linking)
VI  - 30
IP  - 11
DP  - 1995 Nov
TI  - Differences in the intrinsic capacity of peripheral blood mononuclear cells to 
      produce tumor necrosis factor alpha and beta in patients with inflammatory bowel 
      disease and healthy controls.
PG  - 1095-100
AB  - BACKGROUND: Tumor necrosis factor alpha (TNF alpha) and beta (TNF beta) appear to 
      play an important role in the regulation of the inflammatory response. The aim of 
      the present study was to investigate the intrinsic capacity of peripheral blood 
      mononuclear cells (PBMC) to produce these cytokines. METHODS: PBMC from 41 
      patients with Crohn's disease (CD), with ulcerative colitis (UC), and 23 healthy 
      controls (HC) were cultured for 48 h in the presence of the T-cell activators 
      anti-CD3 and anti-CD28. Biologically active total TNF (TNF alpha and beta), TNF 
      alpha, and TNF beta production were measured using a bioassay for biologically 
      active TNF and specific TNF alpha and TNF beta enzyme-linked immunosorbent 
      assays. RESULTS: Large interindividual differences in TNF production were 
      observed. Production of biologically active TNF after T-cell stimulation was 
      significantly decreased in UC patients as compared with HC and CD patients 
      (median, 337 U/ml, 800 U/ml, and 1050 U/ml, respectively). Stimulated TNF alpha 
      production in UC patients (median, 432 U/ml) and in CD patients (median, 537 
      U/ml) did not differ statistically significantly from HC (median, 730 U/ml) as 
      compared with HC and UC patients(median, 800 U/ml and 837 U/ml, respectively). 
      CONCLUSIONS: These findings support the concept that UC and CD are homogeneous, 
      clearly distinguishable disease entities but rather a heterogeneous group of 
      diseases. Studies directed to assess the immunogenetic background of these 
      different disease manifestations in IBD are underway.
FAU - Bouma, G
AU  - Bouma G
AD  - Dept. of Gastroenterology, Free University Hospital, Amsterdam, The Netherlands.
FAU - Oudkerk Pool, M
AU  - Oudkerk Pool M
FAU - Scharenberg, J G
AU  - Scharenberg JG
FAU - Kolkman, J J
AU  - Kolkman JJ
FAU - von Blomberg, B M
AU  - von Blomberg BM
FAU - Scheper, R J
AU  - Scheper RJ
FAU - Meuwissen, S G
AU  - Meuwissen SG
FAU - Pena, A S
AU  - Pena AS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Gastroenterol
JT  - Scandinavian journal of gastroenterology
JID - 0060105
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cells, Cultured
MH  - Colitis, Ulcerative/*blood/drug therapy/immunology
MH  - Crohn Disease/*blood/drug therapy/immunology
MH  - Female
MH  - Humans
MH  - Leukocytes, Mononuclear/immunology/*metabolism
MH  - Lymphotoxin-alpha/*biosynthesis
MH  - Male
MH  - Middle Aged
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.3109/00365529509101613 [doi]
PST - ppublish
SO  - Scand J Gastroenterol. 1995 Nov;30(11):1095-100. doi: 10.3109/00365529509101613.