PMID- 8579128
OWN - NLM
STAT- MEDLINE
DCOM- 19960311
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 148
IP  - 2
DP  - 1996 Feb
TI  - Sequestration of inhaled particulate antigens by lung phagocytes. A mechanism for 
      the effective inhibition of pulmonary cell-mediated immunity.
PG  - 657-66
AB  - Dendritic cells (DCs) have emerged as the dominant antigen-presenting cells 
      (APCs) of the lung, playing a vital role in the induction of cell-mediated 
      immunity to inhaled antigens. We have previously demonstrated that an airway 
      challenge with the soluble antigen hen egg lysozyme yields rapid acquisition of 
      specific antigen-presenting cell activity by purified pulmonary DCs and a 
      cell-mediated immune response in the lung upon secondary challenge. To examine 
      how a particulate antigen leads to a cell-mediated response in vivo, graded 
      concentrations of heat-killed Listeria (HKL) were injected intratracheally into 
      Lewis rats. The bacteria were rapidly ingested by lung macrophages and 
      polymorphonuclear leukocytes. The ability of purified pulmonary DCs pulsed in 
      vivo by an airway challenge with HKL to subsequently stimulate HKL-specific 
      responses ex vivo showed a threshold response, requiring a dose in excess of 
      10(9) organisms/rat. By contrast, all dosages of HKL yielded specific 
      sensitization of lymphocytes in the draining bilar nodes. Pulmonary DCs purified 
      from rats after a secondary in vivo airway challenge with HKL at day 14 were 
      ineffective antigen-presenting cells except at high dosages of antigen. The 
      generation of cell-mediated pulmonary inflammation paralleled the 
      antigen-presenting cell activity of pulmonary DCs and was observed only at high 
      antigen dosages. Hen egg lysozyme immobilized onto polystyrene beads and injected 
      intratracheally yielded comparable results to those observed with HKL. We suggest 
      that a pulmonary cellular immune response is generated to an inhaled particulate 
      antigen when the protective phagocytic capacities of the lung are exceeded and 
      antigen is able to interact directly with interstitial DCs. The diversion of 
      particulate antigens by pulmonary phagocytes may help to limit undesirable 
      pulmonary inflammation while allowing the generation of antigen-specific immune 
      lymphocytes in vivo.
FAU - MacLean, J A
AU  - MacLean JA
AD  - Department of Pathology, Massachusetts General Hospital, Boston 02114, USA.
FAU - Xia, W
AU  - Xia W
FAU - Pinto, C E
AU  - Pinto CE
FAU - Zhao, L
AU  - Zhao L
FAU - Liu, H W
AU  - Liu HW
FAU - Kradin, R L
AU  - Kradin RL
LA  - eng
GR  - AI01245/AI/NIAID NIH HHS/United States
GR  - HL48385/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Antigens, Bacterial)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/*immunology
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Immunity, Cellular
MH  - Inflammation/immunology
MH  - Listeria monocytogenes/immunology
MH  - Lung/*immunology
MH  - Macrophages, Alveolar/*immunology
MH  - Muramidase/immunology
MH  - Neutrophils/*immunology
MH  - Phagocytes/immunology/*pathology
MH  - *Phagocytosis
MH  - Rats
MH  - Rats, Inbred Lew
MH  - T-Lymphocytes/immunology
PMC - PMC1861667
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
PMCR- 1996/08/01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
PHST- 1996/08/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1996 Feb;148(2):657-66.