PMID- 8579756
OWN - NLM
STAT- MEDLINE
DCOM- 19960315
LR  - 20190920
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 34
IP  - 4
DP  - 1995 Oct
TI  - Human leukocyte antigen DQ alpha sharing is not increased in couples with 
      recurrent miscarriage.
PG  - 209-12
AB  - PROBLEM: The results regarding human leukocyte antigen (HLA) DQ alpha allele 
      sharing in recurrent miscarriage couples are conflicting. The purpose of this 
      study was to determine the frequency of HLA DQ alpha allele sharing in our 
      unexplained recurrent spontaneous abortion (RSA) patients using modern DNA 
      analytical techniques. METHODS: DNA was extracted from whole blood samples of 1) 
      51 couples with at least three miscarriages, and 2) 43 fertile couples (with at 
      least seven children and no known history of recurrent miscarriage). The 
      polymerase chain reaction (PCR) was used to amplify the second exon of the HLA DQ 
      alpha locus on chromosome 6. Genotypes were identified by allele specific 
      hybridization with 12 sequence-specific oligonucleotide probes. RESULTS: 47% of 
      recurrent miscarriage couples and 35% of fertile couples shared no alleles. 47% 
      of recurrent miscarriage couples compared to 58% of fertile couples shared one 
      allele, and 6% of recurrent miscarriage couples and 7% of fertile couples shared 
      two alleles. CONCLUSIONS: Reproductive partners with unexplained recurrent 
      pregnancy loss have no increased frequency of HLA DQ alpha allele sharing. It is 
      unlikely that HLA DQ alpha genotyping will be helpful in the management of 
      patients with RSA.
FAU - Dizon-Townson, D
AU  - Dizon-Townson D
AD  - Department of Obstetrics and Gynecology, University of Utah School of Medicine, 
      Salt Lake City, USA.
FAU - Nelson, L
AU  - Nelson L
FAU - Scott, J R
AU  - Scott JR
FAU - Branch, D W
AU  - Branch DW
FAU - Ward, K
AU  - Ward K
LA  - eng
GR  - M01-RR00064/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (HLA-DQ Antigens)
SB  - IM
MH  - Abortion, Habitual/*genetics
MH  - Adult
MH  - Alleles
MH  - Female
MH  - Fertility/genetics
MH  - *Gene Frequency
MH  - Genotype
MH  - HLA-DQ Antigens/*genetics
MH  - Humans
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Spouses/statistics & numerical data
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1111/j.1600-0897.1995.tb00942.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1995 Oct;34(4):209-12. doi: 
      10.1111/j.1600-0897.1995.tb00942.x.