PMID- 8580165
OWN - NLM
STAT- MEDLINE
DCOM- 19960315
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 7
IP  - 4
DP  - 1995 Oct
TI  - Persistence of porcine reproductive and respiratory syndrome virus in serum and 
      semen of adult boars.
PG  - 456-64
AB  - Four seronegative adult boars were intranasally inoculated with porcine 
      reproductive and respiratory syndrome virus (PRRSV) isolate VR-2332. Serum and 
      semen were collected 2-3 times weekly for over 100 days postinoculation (DPI). 
      Serum samples were assayed for PRRSV by virus isolation (VI) and a polymerase 
      chain reaction (PCR) and screened for antibodies to PRRSV using the indirect 
      fluorescent antibody (IFA) and virus neutralization (VN) tests. Semen was assayed 
      for PRRSV RNA by PCR. Virus and viral RNA was detected in the serum of all boars 
      within 1 DPI by Vi and/or PCR. However, VI results indicated that viremia was 
      transient and occurred from 1 to 9 DPI. Viral RNA was detected in serum from 1 to 
      31 DPI. In the acute stage of the infection, PRRSV RNA was detected in serum by 
      PCR prior to the presence of viral RNA in semen. The PRRSV RNA was detected in 
      semen as early as 3 DPI and persisted for 25 DPI in 2 of the boars and 56 and 92 
      DPI in the remaining 2 boars. Detection of PRRSV RNA in semen occurred 2-8 and 
      28-35 days prior to the detection of antibodies by IFA and VN, respectively. 
      PRRSV was isolated from the bulbourethral gland of the boar that shed viral RNA 
      in semen for 92 DPI. These results suggest that PRRSV RNA can be detected by PCR 
      in boar serum and semen, and may persist for variable periods of time. Viremia 
      and the serologic status of the boar are not adequate indicators of when PRRSV or 
      PRRSV RNA is being shed in the semen. Preliminary findings also indicated that 
      neither shipping stress nor reinoculation with homologous PRRSV resulted in 
      viremia or viral RNA shedding in semen.
FAU - Christopher-Hennings, J
AU  - Christopher-Hennings J
AD  - Department of Veterinary Science, South Dakota State University, Brookings 57007, 
      USA.
FAU - Nelson, E A
AU  - Nelson EA
FAU - Hines, R J
AU  - Hines RJ
FAU - Nelson, J K
AU  - Nelson JK
FAU - Swenson, S L
AU  - Swenson SL
FAU - Zimmerman, J J
AU  - Zimmerman JJ
FAU - Chase, C L
AU  - Chase CL
FAU - Yaeger, M J
AU  - Yaeger MJ
FAU - Benfield, D A
AU  - Benfield DA
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Arterivirus/isolation & purification
MH  - Arterivirus Infections/diagnosis/*veterinary
MH  - Base Sequence
MH  - Biological Assay/methods
MH  - Blood/*virology
MH  - DNA Primers
MH  - Fluorescent Antibody Technique, Indirect
MH  - Male
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Open Reading Frames
MH  - Organ Specificity
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Viral/analysis
MH  - Semen/*virology
MH  - Swine
MH  - *Swine Diseases
MH  - Syndrome
MH  - Virus Shedding
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1177/104063879500700406 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 1995 Oct;7(4):456-64. doi: 10.1177/104063879500700406.