PMID- 8580375
OWN - NLM
STAT- MEDLINE
DCOM- 19960321
LR  - 20071115
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 7
IP  - 6
DP  - 1995 Aug
TI  - Expression of monocyte chemoattractant protein-1 in vascular tissue.
PG  - 575-9
AB  - We investigated monocyte chemoattractant protein-1 (MCP-1) mRNA expression in rat 
      vascular smooth muscle cells (VSMC) and in human atherosclerotic arteries to test 
      the involvement of MCP-1 in the pathogenesis of atherosclerosis. In Northern blot 
      analysis, MCP-1 mRNA expression was not observed in unstimulated cultured rat 
      vascular smooth muscle cells (VSMC), but its expression was clearly observed by 
      exposure to tumour necrosis factor-alpha (100 U/ml) for 2-6 h. Mitogen-activated 
      protein kinase activity in VSMC incubated in serum-free culture medium was 
      increased by exposure to 0.5% fetal bovine serum, while the effect was 
      significantly suppressed in the presence of MCP-1 (100 ng/ml). We then evaluated 
      MCP-1 mRNA expression in atherosclerotic arteries obtained from 12 patients 
      undergoing bypass revascularization through reverse transcription-polymerase 
      chain reaction analysis and observed MCP-1 mRNA expression in all atherosclerotic 
      arteries studied. These results support the premise that MCP-1 is secreted by 
      VSMC in atherosclerotic plaques as well as by endothelial cells and macrophages 
      and contributes to the pathogenesis of atherosclerosis.
FAU - Seino, Y
AU  - Seino Y
AD  - Department of Cardiology, Jichi Medical School, Tochigi, Japan.
FAU - Ikeda, U
AU  - Ikeda U
FAU - Takahashi, M
AU  - Takahashi M
FAU - Hojo, Y
AU  - Hojo Y
FAU - Irokawa, M
AU  - Irokawa M
FAU - Kasahara, T
AU  - Kasahara T
FAU - Shimada, K
AU  - Shimada K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Arteriosclerosis/etiology/metabolism/*physiopathology
MH  - Base Sequence
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Cattle
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Muscle, Smooth, Vascular/cytology/*metabolism
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - S1043-4666(85)70078-6 [pii]
AID - 10.1006/cyto.1995.0078 [doi]
PST - ppublish
SO  - Cytokine. 1995 Aug;7(6):575-9. doi: 10.1006/cyto.1995.0078.