PMID- 8581024
OWN - NLM
STAT- MEDLINE
DCOM- 19960321
LR  - 20061115
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 26
IP  - 5-6
DP  - 1995
TI  - Use of the polymerase chain reaction (PCR) to differentiate serologically similar 
      viral haemorrhagic septicaemia (VHS) virus isolates from Europe and America.
PG  - 464-9
AB  - Since 1988, VHSV (viral haemorrhagic septicaemia virus) has occasionally been 
      isolated from salmonids and marine fish in USA. The isolates are so far 
      serologically indistinguishable from the European VHSV reference strain F1. 
      However, the nucleotide sequence of an American isolate (Makah) revealed a unique 
      20 nucleotide sequence in close proximity to the N gene. This sequence is not 
      present in the European VHSV isolates. Through the use of PCR (polymerase chain 
      reaction) and specific primer sets for the amplification of N gene fragments, it 
      is now possible to distinguish between the European and American isolates within 
      half a day.
FAU - Einer-Jensen, K
AU  - Einer-Jensen K
AD  - Danish Veterinary Laboratory, Aarhus N, Denmark.
FAU - Olesen, N J
AU  - Olesen NJ
FAU - Lorenzen, N
AU  - Lorenzen N
FAU - Jorgensen, P E
AU  - Jorgensen PE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (DNA Primers)
RN  - 0 (Viral Core Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Capsid/genetics
MH  - Carps
MH  - Cell Line
MH  - DNA Primers
MH  - Europe
MH  - Fishes/*virology
MH  - Genes, Viral
MH  - Geography
MH  - Molecular Sequence Data
MH  - Oncorhynchus mykiss
MH  - Polymerase Chain Reaction/methods/*veterinary
MH  - Rhabdoviridae/*classification/isolation & purification/physiology
MH  - Serotyping
MH  - United States
MH  - Viral Core Proteins/genetics
MH  - Virus Replication
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PST - ppublish
SO  - Vet Res. 1995;26(5-6):464-9.