PMID- 8581349
OWN - NLM
STAT- MEDLINE
DCOM- 19960321
LR  - 20191210
IS  - 1078-0297 (Print)
IS  - 1078-0297 (Linking)
VI  - 90
IP  - 1
DP  - 1995 Oct
TI  - Calcium antagonistic actions of tetrandrine depend on cell types.
PG  - 59-68
AB  - We examined the effects of tetrandrine (TET) on Ca2+ mobilization in various 
      types of cells using inositol trisphosphate-generating drugs and compared it with 
      those using the microsomal Ca(2+)-ATPase inhibitor thapsigargin (TG) which is a 
      tool for analyzing Ca2+ store-regulated Ca2+ entry (capacitative Ca2+ entry). In 
      rat pheochromocytoma PC12 cells, 100 microM TET abolished high K+ (30 mM)-induced 
      sustained increase in [Ca2+]i and partially inhibited bradykinin (1 
      microM)-induced or TG (100 nM)-induced Ca2+ entry. In NIH/3T3 fibroblasts, 100 
      microM TET abolished Ca2+ entry induced by bombesin (1 microM) or TG (100 nM). In 
      rat glioma C6 cells, the addition of 100 microM TET reduced the sustained 
      elevation of [Ca2+]i induced by endothelin 1 (10 nM) or TG (100 nM) declining to 
      the resting level. In rat parotid acinar cells, 100 microM TET abolished a 
      sustained increase in [Ca2+]i induced by carbachol (100 microM) or TG (100 nM). 
      In human leukemia T-cell line Jurkat, 100 microM TET did not inhibit Ca2+ entry 
      evoked by the anti-CD3 antibody OKT3 (10 micrograms/ml) or TG (100 nM). The 
      present results suggest that the action of TET on Ca2+ entry is dependent on cell 
      types.
FAU - Takemura, H
AU  - Takemura H
AD  - Department of Pharmacology, School of Medicine, Sapporo Medical University, 
      Japan.
FAU - Kwan, C Y
AU  - Kwan CY
FAU - Ohshika, H
AU  - Ohshika H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Res Commun Mol Pathol Pharmacol
JT  - Research communications in molecular pathology and pharmacology
JID - 9437512
RN  - 0 (Alkaloids)
RN  - 0 (Benzylisoquinolines)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Terpenes)
RN  - 132-06-9 (Inosine Triphosphate)
RN  - 29EX23D5AJ (tetrandrine)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - RWP5GA015D (Potassium)
RN  - S8TIM42R2W (Bradykinin)
SB  - IM
MH  - Alkaloids/*pharmacology
MH  - Animals
MH  - *Benzylisoquinolines
MH  - Bradykinin/pharmacology
MH  - Calcium Channel Blockers/*pharmacology
MH  - Calcium-Transporting ATPases/antagonists & inhibitors
MH  - Cell Line
MH  - Enzyme Inhibitors/pharmacology
MH  - Fibroblasts/drug effects/metabolism
MH  - Humans
MH  - Inosine Triphosphate/biosynthesis
MH  - PC12 Cells
MH  - Potassium/pharmacology
MH  - Rats
MH  - Terpenes/pharmacology
MH  - Thapsigargin
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
PST - ppublish
SO  - Res Commun Mol Pathol Pharmacol. 1995 Oct;90(1):59-68.