PMID- 8586775
OWN - NLM
STAT- MEDLINE
DCOM- 19960328
LR  - 20190909
IS  - 0731-5724 (Print)
IS  - 0731-5724 (Linking)
VI  - 14
IP  - 3
DP  - 1995 Jun
TI  - Effect of N-3 polyunsaturated fatty acid supplemented diet on neutrophil-mediated 
      ileal permeability and neutrophil function in the rat.
PG  - 258-63
AB  - RATIONALE AND OBJECTIVE: Fish oil, rich in n-3 polyunsaturated fatty acids, can 
      alter leukotriene production and hence neutrophil function, factors which may be 
      important in the inflammation of Crohn's disease (CD). Therefore we studied the 
      effect of dietary PUFA on neutrophil mediated ileal inflammation and neutrophil 
      function in the rat. METHODS: Animals were ad libitum-fed pellet diets containing 
      9.5% fish oil (menhaden oil, rich in n-3 PUFA) with 0.5% safflower oil, 10% 
      safflower oil (rich in n-6 PUFA) or standard chow (6% fat) for 50 days prior to 
      the study. Weight and circulating leukocyte and total neutrophil counts were 
      identical in all three groups. Neutrophil mediated ileal inflammation induced by 
      formyl-methionyl-leucyl-phenylalanine (fMLP) perfusion was evaluated by measuring 
      macromolecular uptake of radiolabelled dextran (MW 70,000) and changes in mucosal 
      neutrophil infiltration. RESULTS: The fish oil diet group showed no difference in 
      fMLP-induced permeability changes relative to the Chow Control group. However, 
      the Safflower Oil supplemented diet group had a reduced permeability response (p 
      < 0.01). Mirroring the permeability changes, there was diminished mucosal 
      neutrophil infiltration in the Safflower Oil group following ileal perfusion with 
      fMLP (< .005). Chemotaxis and chemiluminescence, two important neutrophil 
      functions, were also significantly suppressed in the Safflower Oil animals (p < 
      0.05). CONCLUSIONS: The failure of a n-3 PUFA enriched diet to diminish the ileal 
      inflammatory response to a bacterial peptide and suppress neutrophil function in 
      the rat suggests such therapy would not be expected to be highly successful in 
      CD. However, it requires confirmation in man, especially under the more 
      complicated inflammatory conditions found in CD. On the other hand, the decreased 
      neutrophil mediated responses with a high linoleic acid (n-6 PUFA) diet warrant 
      further investigation.
FAU - Chawla, A
AU  - Chawla A
AD  - Department of Pediatrics, North Shore University Hospital-Cornell University 
      Medical College, Manhasset, New York, USA.
FAU - Karl, P I
AU  - Karl PI
FAU - Fisher, S E
AU  - Fisher SE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Am Coll Nutr
JT  - Journal of the American College of Nutrition
JID - 8215879
RN  - 0 (Dextrans)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Leukotrienes)
RN  - 0 (Linoleic Acids)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9KJL21T0QJ (Linoleic Acid)
SB  - IM
MH  - Animals
MH  - Chemotaxis, Leukocyte
MH  - Crohn Disease/metabolism/pathology/physiopathology
MH  - Dextrans/pharmacokinetics
MH  - Disease Models, Animal
MH  - Fatty Acids, Omega-3/administration & dosage/*pharmacology
MH  - Food, Fortified
MH  - Ileum/cytology/*metabolism/physiology
MH  - Leukotrienes/metabolism
MH  - Linoleic Acid
MH  - Linoleic Acids/pharmacology
MH  - Luminescent Measurements
MH  - Male
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/*physiology
MH  - Permeability
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1080/07315724.1995.10718505 [doi]
PST - ppublish
SO  - J Am Coll Nutr. 1995 Jun;14(3):258-63. doi: 10.1080/07315724.1995.10718505.