PMID- 8587154
OWN - NLM
STAT- MEDLINE
DCOM- 19960325
LR  - 20190920
IS  - 0140-7783 (Print)
IS  - 0140-7783 (Linking)
VI  - 18
IP  - 5
DP  - 1995 Oct
TI  - Pharmacokinetics of enrofloxacin after single intravenous, intramuscular and 
      subcutaneous injections in lactating cows.
PG  - 357-62
AB  - Five Ayrshire cows were given enrofloxacin (5 mg/kg body weight) intravenously 
      (i.v.), intramuscularly (i.m.) and subcutaneously (s.c.). The antimicrobial 
      activity was measured in milk and serum samples using the agar-diffusion 
      technique. High-performance liquid chromatography (HPLC) assay was used to study 
      the extent of metabolism of enrofloxacin to ciprofloxacin. Analysis of the serum 
      concentration-time data was based on statistical moment theory. Mean t1/2 beta of 
      antimicrobial activity in serum was 1.7, 5.9 and 5.6 h after i.v., i.m. and s.c. 
      administration, respectively. Both i.m. and s.c. routes were associated with a 
      marked flip-flop phenomenon. Based on HPLC analysis of serum samples, the 
      half-lives of enrofloxacin and ciprofloxacin were approximately the same. A 
      marked proportion of enrofloxacin was metabolized to ciprofloxacin. The 
      enrofloxacin fraction bound in vitro to serum proteins was 36-45%. About 0.2% of 
      the total enrofloxacin dose was found in milk during the first 24 h and the 
      amount transferred did not depend on the route of administration. Based on the 
      HPLC data, enrofloxacin concentration in milk was parallel to that in serum, 
      while ciprofloxacin was concentrated in milk. After i.v. injection, the peak 
      concentration of enrofloxacin in milk was reached between 0.7 and 1.3 h but 
      occurred much later for ciprofloxacin (tmax 5-8 h). After i.m. and s.c. 
      administration the concentration-time curves for both enrofloxacin and 
      ciprofloxacin in milk were shallow and there were no obvious peaks.
FAU - Kaartinen, L
AU  - Kaartinen L
AD  - Department of Clinical Sciences, College of Veterinary Medicine, Helsinki, 
      Finland.
FAU - Salonen, M
AU  - Salonen M
FAU - Alli, L
AU  - Alli L
FAU - Pyorala, S
AU  - Pyorala S
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Vet Pharmacol Ther
JT  - Journal of veterinary pharmacology and therapeutics
JID - 7910920
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Fluoroquinolones)
RN  - 0 (Quinolones)
RN  - 3DX3XEK1BN (Enrofloxacin)
RN  - 5E8K9I0O4U (Ciprofloxacin)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents/administration & dosage/*pharmacokinetics/pharmacology
MH  - Cattle/*metabolism
MH  - Chromatography, High Pressure Liquid/veterinary
MH  - Ciprofloxacin/blood
MH  - Computer Simulation
MH  - Enrofloxacin
MH  - Escherichia coli/drug effects
MH  - Female
MH  - *Fluoroquinolones
MH  - Injections, Intramuscular/veterinary
MH  - Injections, Intravenous/veterinary
MH  - Injections, Subcutaneous/veterinary
MH  - Lactation/metabolism
MH  - Microbial Sensitivity Tests/veterinary
MH  - Milk/chemistry/microbiology
MH  - Protein Binding/drug effects
MH  - Quinolones/administration & dosage/*pharmacokinetics/pharmacology
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1111/j.1365-2885.1995.tb00604.x [doi]
PST - ppublish
SO  - J Vet Pharmacol Ther. 1995 Oct;18(5):357-62. doi: 
      10.1111/j.1365-2885.1995.tb00604.x.