PMID- 8587857
OWN - NLM
STAT- MEDLINE
DCOM- 19960327
LR  - 20190905
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 15
IP  - 10
DP  - 1995 Oct
TI  - Flow sorting of fetal erythroblasts using intracytoplasmic anti-fetal 
      haemoglobin: preliminary observations on maternal samples.
PG  - 897-905
AB  - Monoclonal antibody to fetal haemoglobin (alpha 2 gamma 2) has been proposed as a 
      fetal-specific reagent. We developed an intracellular staining protocol that 
      combines fluorescein isothiocyanate or phycoerythrin conjugated anti-gamma with 
      the DNA binding dye Hoechst 33342 to identify and flow sort fetal erythroblasts 
      from maternal blood. Our preliminary observations on anti-gamma-positive cells 
      sorted from four different pregnant women are described here, using fluorescence 
      in situ hybridization (FISH) with chromosome-specific probes to identify fetal 
      cells. Our data demonstrate that far fewer candidate fetal cells are sorted with 
      this protocol than by current cell surface staining methods that employ the 
      monoclonal antibody CD71. This results in increased fetal cell sorting purities. 
      With this protocol, standard FISH techniques require modification due to the 
      rigorous fixation with 4 per cent paraformaldehyde. Our initial data indicate the 
      promise of this approach.
FAU - Zheng, Y L
AU  - Zheng YL
AD  - Department of Pediatrics, New England Medical Center, Boston, MA 02111, USA.
FAU - Demaria, M
AU  - Demaria M
FAU - Zhen, D
AU  - Zhen D
FAU - Vadnais, T J
AU  - Vadnais TJ
FAU - Bianchi, D W
AU  - Bianchi DW
LA  - eng
GR  - P01 HD 18658/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 9034-63-3 (Fetal Hemoglobin)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Adult
MH  - *Antibodies, Monoclonal
MH  - Cell Separation/*methods
MH  - Cytoplasm
MH  - DNA Probes
MH  - Erythroblasts/*cytology
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Fetal Hemoglobin/*immunology
MH  - Flow Cytometry/*methods
MH  - Fluorescein-5-isothiocyanate
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Pregnancy
MH  - X Chromosome
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1002/pd.1970151004 [doi]
PST - ppublish
SO  - Prenat Diagn. 1995 Oct;15(10):897-905. doi: 10.1002/pd.1970151004.