PMID- 8587862
OWN - NLM
STAT- MEDLINE
DCOM- 19960327
LR  - 20190905
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 15
IP  - 10
DP  - 1995 Oct
TI  - Detection of fetal cells in transcervical samples and prenatal diagnosis of 
      chromosomal abnormalities.
PG  - 943-9
AB  - Transcervical samples collected by lavage, aspiration, and cytobrush from women 
      between 6 and 13 weeks of gestation were tested for the presence of fetal cells 
      using fluorescence in situ hybridization (FISH) with probes for chromosomes X, Y, 
      1, and 21, and by polymerase chain reaction (PCR) amplification of DNA sequences 
      derived from chromosomes X, Y, and 21. With a few exceptions, a good correlation 
      was observed between the results of sexing the fetuses using FISH or PCR on 
      transcervical cell (TCC) samples retrieved by lavage and those obtained by 
      testing fetal (placental) tissue. In a comparative study between TCC samples 
      collected by lavage or cytobrush, the sex of the fetus was correctly diagnosed by 
      PCR amplification of a Y-derived DNA sequence. Variable results were observed 
      with samples obtained by aspiration, mainly because this procedure was found to 
      be more prone to failure to remove thick mucus without previous injection of 
      physiological saline. Chromosome 21-derived small tandem repeats (STRs) of fetal 
      origin were successfully detected in about 40 per cent of TCC samples recovered 
      by lavage. Two cases of chromosomal abnormalities, one of trisomy 21 and one of 
      triploidy, were detected in TCC samples in the course of our investigations.
FAU - Adinolfi, M
AU  - Adinolfi M
AD  - Department of Obstetrics and Gynaecology, University College London Medical 
      School, U.K.
FAU - Sherlock, J
AU  - Sherlock J
FAU - Tutschek, B
AU  - Tutschek B
FAU - Halder, A
AU  - Halder A
FAU - Delhanty, J
AU  - Delhanty J
FAU - Rodeck, C
AU  - Rodeck C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Cell Separation
MH  - Cervix Uteri
MH  - DNA Probes
MH  - Female
MH  - Fetus/*cytology
MH  - Gestational Age
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Sex Chromosome Aberrations/*diagnosis
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1002/pd.1970151009 [doi]
PST - ppublish
SO  - Prenat Diagn. 1995 Oct;15(10):943-9. doi: 10.1002/pd.1970151009.