PMID- 8587863
OWN - NLM
STAT- MEDLINE
DCOM- 19960327
LR  - 20190905
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 15
IP  - 10
DP  - 1995 Oct
TI  - Isolation of fetal cells from transcervical samples by micromanipulation: 
      molecular confirmation of their fetal origin and diagnosis of fetal aneuploidy.
PG  - 951-60
AB  - Transcervical cell (TCC) samples have been shown to contain fetal cells amenable 
      to molecular analysis. However, the presence of 'contaminating' maternal cells 
      limits their use for prenatal diagnoses. In this report we show that clumps of 
      fetal cells can be isolated from transcervical samples by micromanipulation and 
      tested by fluorescence in situ hybridization (FISH) and polymerase chain reaction 
      (PCR). Out of 129 clumps, isolated from mucus aspirates and transcervical lavages 
      from 29 patients, 29 clumps from 11 patients were found to be exclusively of 
      fetal origin as judged by the detection of chromosome 21-specific polymorphic DNA 
      markers and Y-derived DNA sequences by PCR and FISH. One case of a male triploid 
      fetus, diagnosed by the analysis of TCC samples obtained by mucus aspiration and 
      lavage, was confirmed by testing clumps of cells isolated by micromanipulation.
FAU - Tutschek, B
AU  - Tutschek B
AD  - Department of Obstetrics and Gynaecology, University College London, U.K.
FAU - Sherlock, J
AU  - Sherlock J
FAU - Halder, A
AU  - Halder A
FAU - Delhanty, J
AU  - Delhanty J
FAU - Rodeck, C
AU  - Rodeck C
FAU - Adinolfi, M
AU  - Adinolfi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Aneuploidy
MH  - Cell Separation/*methods
MH  - DNA Probes
MH  - Female
MH  - Gestational Age
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Micromanipulation
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1002/pd.1970151010 [doi]
PST - ppublish
SO  - Prenat Diagn. 1995 Oct;15(10):951-60. doi: 10.1002/pd.1970151010.