PMID- 8587864
OWN - NLM
STAT- MEDLINE
DCOM- 19960327
LR  - 20190905
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 15
IP  - 10
DP  - 1995 Oct
TI  - Uterine cavity lavage: adding FISH to conventional cytogenetics for embryonic 
      sexing and diagnosing common chromosomal aberrations.
PG  - 961-5
AB  - This study was undertaken to examine the efficacy for early prenatal diagnosis of 
      uterine cavity lavage at the level of the internal os and to assess the rate of 
      maternal contamination. In phase I, uterine cavity lavage was performed in 38 
      women scheduled for pregnancy termination between 6 and 12 weeks. In addition to 
      short- and long-term cultures, one-colour FISH (fluorescence in situ 
      hybridization) with Y and X probes was used for fetal sexing. Two-colour FISH was 
      used in all known male fetuses for the assessment of maternal contamination. In 
      phase II, lavage was performed on 16 women. Fetal sex was diagnosed with direct 
      labelled X and Y probes and common numerical chromosomal aberration was attempted 
      with 18 and 21 direct labelled probes. Fetal sexing was successful in all cases 
      in phases I and II. Out of 34 patients in which tissue was obtained, only FISH 
      was done in six. Long-term cell cultures were successful in the other 28 cases, 
      but complete karyotyping in 19 (56 per cent). No chromosomal aberration was found 
      with the direct labelled probes 18 and 21 in FISH. Maternal contamination was 
      assessed to be 5-10 per cent. This simple and easy-to-master technique is very 
      effective in obtaining fetal cells early in pregnancy for genetic diagnosis, 
      especially by FISH. However, the safety of the procedure must be tested in 
      ongoing pregnancies.
FAU - Ishai, D
AU  - Ishai D
AD  - Genetics Institute, Meir General Hospital, Kfar Saba, Haifa, Israel.
FAU - Amiel, A
AU  - Amiel A
FAU - Diukman, R
AU  - Diukman R
FAU - Cogan, O
AU  - Cogan O
FAU - Lichtenstein, Z
AU  - Lichtenstein Z
FAU - Abramovici, H
AU  - Abramovici H
FAU - Fejgin, M D
AU  - Fejgin MD
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cells, Cultured
MH  - Cervix Uteri
MH  - *Chromosome Aberrations
MH  - DNA Probes
MH  - Female
MH  - Gestational Age
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Placenta
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Sex Chromosomes
MH  - Sex Determination Analysis/*methods
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1002/pd.1970151011 [doi]
PST - ppublish
SO  - Prenat Diagn. 1995 Oct;15(10):961-5. doi: 10.1002/pd.1970151011.