PMID- 8588920
OWN - NLM
STAT- MEDLINE
DCOM- 19960322
LR  - 20131121
IS  - 0736-6205 (Print)
IS  - 0736-6205 (Linking)
VI  - 19
IP  - 5
DP  - 1995 Nov
TI  - Simultaneous detection of TDT-mediated dUTP-biotin nick end-labeling 
      (TUNEL)-positive cells and multiple immunohistochemical markers in single tissue 
      sections.
PG  - 800-5
AB  - We have modified the terminal deoxynucleotidyl transferase (TDT)-mediated 
      dUTP-biotin nick end-labeling (TUNEL) method to permit the immunogold-silver 
      intensification detection of apoptotic cells in tissue sections. Such sections 
      can subsequently be processed for multi-labeling fluorescence microscopy, thus 
      permitting the simultaneous detection of silver-positive apoptotic cells and 
      three additional fluorescent signals. In this report, we combined TUNEL labeling 
      with the fluorescent detection of bromodeoxyuridine-positive proliferating cells, 
      growth-associated protein-43 (GAP-43) immunoreactive neurons and 
      bisbenzimide-positive pyknotic cells in the embryonic mouse nervous system. This 
      combination of stains allowed the simultaneous detection of proliferative, 
      differentiated and apoptotic cells in the developing nervous system.
FAU - Tornusciolo, D R
AU  - Tornusciolo DR
AD  - Washington University School of Medicine, St. Louis, MO, USA.
FAU - Schmidt, R E
AU  - Schmidt RE
FAU - Roth, K A
AU  - Roth KA
LA  - eng
GR  - AG-10299/AG/NIA NIH HHS/United States
GR  - DK19645/DK/NIDDK NIH HHS/United States
GR  - DK44983/DK/NIDDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
RN  - 0 (Deoxyuracil Nucleotides)
RN  - 1173-82-6 (deoxyuridine triphosphate)
RN  - 6SO6U10H04 (Biotin)
RN  - EC 2.7.7.31 (DNA Nucleotidylexotransferase)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - *Biotin
MH  - Cells, Cultured
MH  - DNA Nucleotidylexotransferase/*metabolism
MH  - Deoxyuracil Nucleotides/*metabolism
MH  - Female
MH  - *Immunohistochemistry
MH  - Mice
MH  - Neurons/physiology
MH  - Pregnancy
MH  - Silver Staining
MH  - Staining and Labeling/*methods
MH  - Telencephalon/*cytology/embryology
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
PST - ppublish
SO  - Biotechniques. 1995 Nov;19(5):800-5.