PMID- 8589675
OWN - NLM
STAT- MEDLINE
DCOM- 19960327
LR  - 20190512
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 4
IP  - 11
DP  - 1995 Nov
TI  - Analysis of chromosome behavior in intact mammalian oocytes: monitoring the 
      segregation of a univalent chromosome during female meiosis.
PG  - 2007-12
AB  - To monitor the behavior of specific chromosomes at various stages of mammalian 
      female meiosis, we have combined immunofluorescence staining and fluorescence in 
      situ hybridization (FISH) on intact oocytes. We have utilized this technique to 
      evaluate the behavior of the single X chromosome in oocytes from XO female mice, 
      providing the first observations on segregation of an achiasmate chromosome 
      during mammalian female meiosis and its effect on the meiotic process. As has 
      been described in other species, we found that the univalent chromosome could 
      either segregate as an intact chromosome to one pole or divide equationally at 
      the first meiotic division. Our results also indicate that the presence of a 
      univalent chromosome causes severe meiotic disruption during mammalian meiosis, 
      affecting the alignment and segregation of other chromosomes in the complement. 
      Despite these meiotic abnormalities, the vast majority of oocytes from XO females 
      were able to resume and successfully complete the first meiotic division. This is 
      in contrast to previous studies of male mice with sex chromosome abnormalities 
      where the presence of a univalent acts to arrest meiosis at metaphase of the 
      first meiotic division. This sex-specific difference in the ability of a cell 
      with a univalent chromosome to initiate anaphase suggests that cell cycle control 
      differs between male and female meiosis and that monitoring of meiotic chromosome 
      behavior is less efficient in the female. The combined use of immunofluorescence 
      staining and FISH on intact oocytes has obvious application to the study of 
      meiotic chromosome non-disjunction in the human female. Simultaneous study of the 
      meiotic cell cycle, protein components of the meiotic apparatus, and 
      chromosome-specific behaviors during mammalian female meiosis provides a new 
      approach to defining age-related changes in the meiotic process that result in 
      increased chromosome malsegregation.
FAU - Hunt, P
AU  - Hunt P
AD  - Department of Genetics, Case Western Reserve University School of Medicine, 
      Cleveland, OH 44106-4955, USA.
FAU - LeMaire, R
AU  - LeMaire R
FAU - Embury, P
AU  - Embury P
FAU - Sheean, L
AU  - Sheean L
FAU - Mroz, K
AU  - Mroz K
LA  - eng
GR  - HD27393/HD/NICHD NIH HHS/United States
GR  - HD31866/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
SB  - IM
MH  - Anaphase
MH  - Animals
MH  - Cell Cycle
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mammals
MH  - Meiosis/*genetics
MH  - Metaphase
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oocytes/*cytology
MH  - Telophase
MH  - *X Chromosome
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1093/hmg/4.11.2007 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1995 Nov;4(11):2007-12. doi: 10.1093/hmg/4.11.2007.