PMID- 8592788
OWN - NLM
STAT- MEDLINE
DCOM- 19960403
LR  - 20150505
IS  - 0201-8470 (Print)
IS  - 0201-8470 (Linking)
VI  - 67
IP  - 2
DP  - 1995 Mar-Apr
TI  - [Cooperative effect of high density lipoproteins and adaptive hormones in 
      chromatin activation in rat liver].
PG  - 64-70
AB  - Fluorescent probing of rat liver chromatin with acridine orange (AO) and ethidium 
      bromide was employed to investigate the rat liver chromatin activation under 
      stress in vivo and in vitro. A significant increase of molar concentration of AO 
      binding sites with chromatin in nuclei suspension was demonstrated after 
      intensive physical exercises and partial hepatectomy. These results reveal 
      chromatin activation under these stress conditions. For the first time the 
      chromatin activation was shown to be a result of the cooperative effect of 
      hydrocortisone and blood plasma high density lipoproteins (HDL). Both HDL2 and 
      HDL3 contribute to this cooperative action. The increase of concentration of AO 
      binding sites and simultaneous increase of the ratio of red to green fluorescence 
      (alpha), which represents the amount of one-stranded nucleic acids, show that 
      chromatin activation in this case is accompanied by the derepression of new genes 
      and also by the enhanced expression of already functioning ones. Adrenaline did 
      not change the amount of AO binding sites but increased index alpha. This 
      evidences for the enhanced expression of already functioning genes. 
      Hydrocortisone-dependent chromatin activation led to the increase of the binding 
      constant of AO with DNA, while molar concentration of binding sites of the probe 
      was practically unaltered. At the same time, cooperative effect of hydrocortisone 
      and HDL did not alter the constants of AO binding, but the concentration of the 
      probe binding sites with DNA was significantly increased.
FAU - Panin, L E
AU  - Panin LE
FAU - Svechnikova, I G
AU  - Svechnikova IG
FAU - Maianskaia, N N
AU  - Maianskaia NN
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Kooperativnyi effekt lipoproteinov vysokoi plotnosti i adaptivnykh gormonov v 
      aktivatsii khromatina pecheni krys.
PL  - Ukraine
TA  - Ukr Biokhim Zh (1978)
JT  - Ukrainskii biokhimicheskii zhurnal (1978)
JID - 7804246
RN  - 0 (Chromatin)
RN  - 0 (Lipoproteins, HDL)
RN  - WI4X0X7BPJ (Hydrocortisone)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - *Adaptation, Physiological
MH  - Animals
MH  - Chromatin/*drug effects/metabolism
MH  - Epinephrine/pharmacology
MH  - Female
MH  - Hydrocortisone/*pharmacology
MH  - Lipoproteins, HDL/*pharmacology
MH  - Liver/*drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Stress, Physiological/*metabolism
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
PST - ppublish
SO  - Ukr Biokhim Zh (1978). 1995 Mar-Apr;67(2):64-70.