PMID- 8593246
OWN - NLM
STAT- MEDLINE
DCOM- 19960408
LR  - 20190920
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 7
IP  - 8
DP  - 1995 Nov
TI  - Effect of essential fatty acid deficiency on G-proteins, cAMP-dependent protein 
      kinase activity and mucin secretion in the rat submandibular salivary glands.
PG  - 773-81
AB  - Studies were conducted to determine whether beta-adrenergic cell signalling is 
      altered in submandibular salivary glands (SMSG) is essential fatty acid (EFA) 
      deficiency. Three groups of rats were fed diets which were deficient in EFA 
      (EFAD), marginally deficient in EFA (MEFAD) or contained sufficient amount of EFA 
      (Control). Rats were killed after 20 wk on diets, SMSG were dissected out and 
      cyclic AMP-dependent protein kinase (PKA) activity was measured. The specific 
      enzyme activities were higher in the homogenates and supernatant fractions of the 
      gland from EFAD and MEFAD rats compared with the controls. The relative levels of 
      guanine nucleotide-binding regulatory proteins (Gs and Gi) were also measured in 
      the SMSG membranes of rats fed the 3 diets. The levels of Gs were significantly 
      higher in the EFAD and MEFAD groups than in the controls. No significant 
      differences were observed in the secretion of trichloroacetic 
      acid-phosphotungstic acid (TCA-PTA) precipitable glycoproteins from the SMSG 
      slices among the 3 dietary groups.
FAU - Alam, S Q
AU  - Alam SQ
AD  - Department of Biochemistry and Molecular Biology, Louisiana State University 
      Medical Center, New Orleans 70119, USA.
FAU - Abdel-Hakim, S M
AU  - Abdel-Hakim SM
FAU - Alam, B S
AU  - Alam BS
FAU - Ibrahim, I Y
AU  - Ibrahim IY
LA  - eng
GR  - DE 05978/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Dietary Fats)
RN  - 0 (Fatty Acids, Essential)
RN  - 0 (Mucins)
RN  - 0 (Receptors, Adrenergic, beta)
RN  - 0 (Salivary Proteins and Peptides)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/metabolism
MH  - Animals
MH  - Body Weight/drug effects
MH  - Cyclic AMP/physiology
MH  - Cyclic AMP-Dependent Protein Kinases/*metabolism
MH  - Dietary Fats/administration & dosage
MH  - Fatty Acids, Essential/*deficiency/metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Male
MH  - Mucins/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Adrenergic, beta/*physiology
MH  - Saliva/metabolism
MH  - Salivary Proteins and Peptides/*metabolism
MH  - Secretory Rate/drug effects
MH  - *Signal Transduction
MH  - Submandibular Gland/*metabolism
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 0898656895020055 [pii]
AID - 10.1016/0898-6568(95)02005-5 [doi]
PST - ppublish
SO  - Cell Signal. 1995 Nov;7(8):773-81. doi: 10.1016/0898-6568(95)02005-5.