PMID- 8593471
OWN - NLM
STAT- MEDLINE
DCOM- 19960411
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 18
IP  - 11
DP  - 1995 Nov
TI  - Conversion of cathepsin E to enzymatic unstable form in gastric cancer cells.
PG  - 1522-5
AB  - A new acid proteinase in human gastric cancer, named medium moving proteinase 
      (Med.P), was found also in a gastric cancer transplanted into nude mouse. 
      However, Med.P disappeared when the samples prepared from gastric cancer tissues 
      were left for over 4 weeks at -80 degrees C, whereas the activity of cathepsin E 
      (CE) increased. When these samples were reduced by dithiothreitol (DTT), Med.P 
      appeared again and the CE activity decreased. These phenomena, revealed by 
      electrophoretic analyses, indicated that Med.P is a monomeric form of CE 
      (mono-CE). At weakly alkaline pH and after heating, mono-CE appeared to be more 
      unstable than CE. These results indicated that CE assume an enzymatically 
      unstable monomeric form in cancer cells.
FAU - Aoki, T
AU  - Aoki T
AD  - Department of Biochemistry, Faculty of pharmaceutical Sciences, Health Sciences 
      University of Hokkaido, Japan.
FAU - Takasaki, T
AU  - Takasaki T
FAU - Furukawa, T
AU  - Furukawa T
FAU - Morikawa, J
AU  - Morikawa J
FAU - Yano, T
AU  - Yano T
FAU - Watabe, H
AU  - Watabe H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Isoenzymes)
RN  - 0 (Sulfhydryl Reagents)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.23.34 (Cathepsin E)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Animals
MH  - Cathepsin E
MH  - Cathepsins/*metabolism
MH  - Dithiothreitol/pharmacology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Endopeptidases/metabolism
MH  - Humans
MH  - Isoenzymes/*metabolism
MH  - Mice
MH  - Mice, Nude
MH  - Stomach Neoplasms/*enzymology
MH  - Sulfhydryl Reagents/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1248/bpb.18.1522 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 1995 Nov;18(11):1522-5. doi: 10.1248/bpb.18.1522.