PMID- 8595864
OWN - NLM
STAT- MEDLINE
DCOM- 19960418
LR  - 20181130
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 135
IP  - 2-3
DP  - 1996 Jan 15
TI  - Comparison of cytosolic levels of calcium and G actin in diffuse and localised 
      adherent Escherichia coli-infected HeLa cells.
PG  - 245-9
AB  - In the present study we compared the intracellular level of free calcium 
      ([Ca2+]i) and monomeric (G)/total (G+F) actin ratio in HeLa cells infected with 
      diffuse (DAEC) and localised adherent Escherichia coli (LAEC). The level of 
      [Ca2+]i was increased in both DAEC- and LAEC-infected HeLa cells. However, 
      studies with EGTA- and dantrolene-treated cells and also suspension of cells in 
      Ca(2+)-free buffer suggested that the rise of [Ca2+]i in DAEC-infected cells was 
      due to the influx of Ca2+ from extracellular medium, whereas Ca2+ mobilisation 
      from the intracellular stores was responsible for the enhancement of [Ca2+]i in 
      LAEC-infected cells. It was also evident that the infection of HeLa cells with 
      DAEC and LAEC caused alteration of G/G+F actin ratio as compared to that of 
      control cells. The ratio was much lower in LAEC-infected cells than that of 
      DAEC-infected ones. Moreover, cytochalasin B inhibited both DAEC and LAEC 
      invasion to HeLa cells, suggesting further the role of microfilaments in the 
      invasion process.
FAU - Karmaker, S
AU  - Karmaker S
AD  - National Institute of Cholera and Enteric Diseases, Calcutta, India.
FAU - Chaudhuri, A G
AU  - Chaudhuri AG
FAU - Ganguly, U
AU  - Ganguly U
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Actins)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Actins/*metabolism
MH  - Bacterial Adhesion
MH  - Calcium/*metabolism
MH  - Cytochalasin B/pharmacology
MH  - Cytosol/metabolism
MH  - Escherichia coli/cytology/*pathogenicity
MH  - HeLa Cells
MH  - Humans
MH  - Ion Transport
EDAT- 1996/01/15 00:00
MHDA- 1996/01/15 00:01
CRDT- 1996/01/15 00:00
PHST- 1996/01/15 00:00 [pubmed]
PHST- 1996/01/15 00:01 [medline]
PHST- 1996/01/15 00:00 [entrez]
AID - 10.1111/j.1574-6968.1996.tb07996.x [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 1996 Jan 15;135(2-3):245-9. doi: 
      10.1111/j.1574-6968.1996.tb07996.x.