PMID- 8595903
OWN - NLM
STAT- MEDLINE
DCOM- 19960416
LR  - 20190905
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 30
IP  - 1
DP  - 1995 Nov 1
TI  - Feature mapping of the HLA class I region: localization of the POU5F1 and TCF19 
      genes.
PG  - 53-8
AB  - The class I region of the human leukocyte antigen (HLA) complex located on 
      chromosome 6p21.3 is gene dense. To define the gene content of the class I 
      region, we are constructing genomic DNA feature maps. Here we report mapping of 
      the POU5F1 and TCF19 genes to an approximately 0.2-Mb region between the HLA-C 
      and the S genes. Localization of these genes was facilitated by subcloning 
      genomic DNA fragments from the 0.2-Mb region into a transposon gamma delta-based 
      vector, selecting transposon-mediated deletions in vivo in Escherichia coli, and 
      sequencing a nested subset chosen for their uniform distribution of deletion 
      endpoints. The POU5F1 and TCF19 genes are located approximately 130 kb telomeric 
      of the HLA-C locus, approximately 0.6 kb apart from each other. Complete 
      sequencing of a 5.5-kb EcoRI fragment containing the TCF19 gene revealed that it 
      is composed of three exons, bounded by consensus splice signals. These 
      experiments illustrate that the transposon-based nested deletion sequencing 
      method provides an easy and efficient approach to feature mapping genomic 
      fragments and to high-resolution analysis of selected subportions.
FAU - Krishnan, B R
AU  - Krishnan BR
AD  - Department of Internal Medicine, Washington University School of Medicine, St. 
      Louis, Missouri 63110, USA.
FAU - Jamry, I
AU  - Jamry I
FAU - Chaplin, D D
AU  - Chaplin DD
LA  - eng
SI  - GENBANK/U27989
SI  - GENBANK/U27990
SI  - GENBANK/U27991
SI  - GENBANK/U27992
SI  - GENBANK/U27993
SI  - GENBANK/U27994
SI  - GENBANK/U27995
SI  - GENBANK/U27996
SI  - GENBANK/U27997
SI  - GENBANK/U27998
SI  - GENBANK/U27999
SI  - GENBANK/U28000
SI  - GENBANK/U28001
SI  - GENBANK/U28002
SI  - GENBANK/U28003
SI  - GENBANK/U28004
GR  - AI15322/AI/NIAID NIH HHS/United States
GR  - AI34580/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Octamer Transcription Factor-3)
RN  - 0 (POU5F1 protein, human)
RN  - 0 (TCF19 protein, human)
RN  - 0 (Transcription Factors)
RN  - EC 2.1.1.- (DNA modification methylase EcoRI)
RN  - EC 2.1.1.72 (Site-Specific DNA-Methyltransferase (Adenine-Specific))
SB  - IM
MH  - Base Sequence
MH  - Chromosome Mapping/*methods
MH  - DNA-Binding Proteins/*genetics
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Octamer Transcription Factor-3
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion
MH  - Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics
MH  - Transcription Factors/*genetics
MH  - Transcription, Genetic
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - S0888754385700080 [pii]
AID - 10.1006/geno.1995.0008 [doi]
PST - ppublish
SO  - Genomics. 1995 Nov 1;30(1):53-8. doi: 10.1006/geno.1995.0008.