PMID- 8595949
OWN - NLM
STAT- MEDLINE
DCOM- 19960416
LR  - 20220310
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 37
IP  - 4
DP  - 1996 Mar
TI  - Effects of axotomy and intraocular administration of NT-4, NT-3, and 
      brain-derived neurotrophic factor on the survival of adult rat retinal ganglion 
      cells. A quantitative in vivo study.
PG  - 489-500
AB  - PURPOSE: To investigate in vivo the survival of retinal ganglion cells (RGC) 4 to 
      14 days after optic nerve (ON) transection alone or in combination wih a single 
      intraocular injection of neurotrophin-4 (NT-4), neurotrophin-3 (NT-3), or 
      brain-derived neurotrophic factor (BDNF). METHODS: In adult rats, RGCs were 
      labeled with fluorogold (FG) applied to their main targets in the brain. Seven 
      days later, the left ON was intraorbitally transected, and, in several groups of 
      animals, the left eye received a sham injection or was injected with 5 
      microliters of 1% bovine serum albumin-phosphate-buffered saline alone or 
      containing 5 micrograms of NT-4, NT-3, or BDNF. Four, 5, 7, 9, 12 and 14 days 
      after ON transection, the retinas were examined under fluorescence microscopy to 
      estimate RGC survival. RESULTS: In control retinas, the mean densities 
      (cells/mm2+/-SEM) of FG-labeled RGCs were 2421+/-55 (n=20). Four days after 
      axotomy, the densities of RGCs were similar to those observed in control retinas, 
      but 5 and 7 days after axotomy, the mean densities had decreased to 2028+/-63 
      (n=6) and 1568+/-50 (n=6) respectively. In the group of retinas with sham 
      injection, with vehicle alone or with NT-3, RGC densities also decreased by 7 
      days to 1261+/-71 (n=5), 1506+/-98 (n=10), and 1474+/-125 (n=4), respectively. 
      However, similar densities to those observed in control retinas were observed 7 
      days after ON transection in the groups of retinas treated with NT-4 (2505+/-91; 
      n=7) or BDNF (2380+/-74; n=7). Fourteen days after axotomy, RGC densities 
      decreased to 521+/-39 (n=10). Comparable densities were found in groups that 
      underwent axotomy and either sham injection (533+/-51; n=5), injection of vehicle 
      (588+/-19; n=10), or NT-3 treatment (634+/-62; n=6). However, at this time, 
      higher densities were observed in the groups treated with NT-4 839+/-39 (n=8) or 
      BDNF 1321+/-120 (n=7). CONCLUSIONS: Axotomy-induced RGC death first appears by 
      day 5 and reaches 80% of the original RGC population by day 12. NT-4 and BDNF 
      administered intraocularly at the time of axotomy exert a neuroprotective effect 
      on axotomy-induced RGC death, thus increasing the population of surviving RGCs 
      and delaying the onset of RGC of axotomy-induced RGC death by approximately 3 
      days. Intraocular administration of NT-3 did not modify the survival of RGCs 
      after injury.
FAU - Peinado-Ramon, P
AU  - Peinado-Ramon P
AD  - Departamento de Oftalmologia, Otorrinolaringologia y Anatomia Patologica, 
      Universidad de Murcia, Spain.
FAU - Salvador, M
AU  - Salvador M
FAU - Villegas-Perez, M P
AU  - Villegas-Perez MP
FAU - Vidal-Sanz, M
AU  - Vidal-Sanz M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Stilbamidines)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Axons/*physiology
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Count
MH  - Cell Death
MH  - Cell Survival/drug effects
MH  - Denervation
MH  - Female
MH  - Fluorescent Dyes
MH  - Injections
MH  - Microscopy, Fluorescence
MH  - Nerve Growth Factors/administration & dosage/*pharmacology
MH  - Nerve Tissue Proteins/administration & dosage/*pharmacology
MH  - Neurotrophin 3
MH  - Optic Nerve/surgery
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retinal Ganglion Cells/*cytology/drug effects
MH  - *Stilbamidines
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1996 Mar;37(4):489-500.