PMID- 8596467
OWN - NLM
STAT- MEDLINE
DCOM- 19960416
LR  - 20190512
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 10
IP  - 6
DP  - 1995 Nov
TI  - Influence of smoking habits on the frequencies of structural and numerical 
      chromosomal aberrations in human peripheral blood lymphocytes using the 
      fluorescence in situ hybridization (FISH) technique.
PG  - 487-95
AB  - The influence of smoking on the spontaneous frequencies of unstable and stable 
      chromosomal aberrations in stimulated lymphocytes using conventional Giemsa 
      staining and fluorescence in situ hybridization (FISH) was studied. The study 
      groups consisted of 12 smokers and 30 nonsmokers. The frequency of translocations 
      was determined using nine different chromosome-specific DNA libraries, 
      representing approximately 50% of the human genome, usually in a combination of 
      two to three chromosomes in one cocktail. Using DNA libraries for specific 
      chromosomes and FISH also made it possible to detect aneuploid cells. No 
      significant difference was observed between smokers and nonsmokers in the 
      frequencies of unstable and stable type of chromosomal aberrations. However, a 
      significantly (P < or = 0.05) higher frequency of hyperploidy was evident in 
      smokers compared with nonsmokers.
FAU - van Diemen, P C
AU  - van Diemen PC
AD  - MGC, Department of Radiation Genetics and Chemical Mutagenesis, State University 
      of Leiden, The Netherlands.
FAU - Maasdam, D
AU  - Maasdam D
FAU - Vermeulen, S
AU  - Vermeulen S
FAU - Darroudi, F
AU  - Darroudi F
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
SB  - IM
MH  - Adult
MH  - Age Distribution
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human
MH  - Diploidy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/*ultrastructure
MH  - Male
MH  - Middle Aged
MH  - Ring Chromosomes
MH  - Sex Distribution
MH  - Smoking/*adverse effects
MH  - Translocation, Genetic
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1093/mutage/10.6.487 [doi]
PST - ppublish
SO  - Mutagenesis. 1995 Nov;10(6):487-95. doi: 10.1093/mutage/10.6.487.