PMID- 8596470
OWN - NLM
STAT- MEDLINE
DCOM- 19960416
LR  - 20190512
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 10
IP  - 6
DP  - 1995 Nov
TI  - Analysis by fluorescence in situ hybridization with a mouse gamma satellite DNA 
      probe of isolated micronuclei induced in mice by two clastogens and two spindle 
      poisons.
PG  - 513-6
AB  - The presence of centromeric DNA was studied in micronuclei isolated from the 
      blood of male ddY mice after five weekly intraperitoneal injections of mitomycin 
      C (MMC), 1-beta-D-arabinofuranosylcytosine (Ara-C), colchicine (COL) or 
      vinblastine sulfate (VBL). In agreement with our earlier findings, about half of 
      the micronuclei isolated from vehicle control mice showed centromere signals as 
      analyzed by fluorescence in situ hybridization (FISH) with a mouse major (gamma) 
      satellite DNA probe. In an earlier experiment with mice acutely exposed to the 
      same chemicals, the clastogens MMC and Ara-C did not reduce the proportion of 
      micronuclei with centromere signals. In the present study, however, MMC and Ara-C 
      decreased the proportion of micronuclei with centromeres. In contrast, the 
      spindle poisons COL and VBL increased the proportion of micronuclei that 
      contained centromeres.
FAU - Maki-Paakkanen, J
AU  - Maki-Paakkanen J
AD  - Division of Environmental Health, National Public Health Institute, Kuopio, 
      Finland.
FAU - Hayashi, M
AU  - Hayashi M
FAU - Suzuki, T
AU  - Suzuki T
FAU - Tanabe, H
AU  - Tanabe H
FAU - Honma, M
AU  - Honma M
FAU - Sofuni, T
AU  - Sofuni T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
RN  - 0 (Mutagens)
RN  - 04079A1RDZ (Cytarabine)
RN  - 50SG953SK6 (Mitomycin)
RN  - 5V9KLZ54CY (Vinblastine)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Animals
MH  - Centromere/drug effects
MH  - Colchicine/toxicity
MH  - Cytarabine/toxicity
MH  - DNA Probes/chemistry/genetics
MH  - DNA, Satellite/chemistry/genetics
MH  - Dose-Response Relationship, Drug
MH  - Erythrocytes/drug effects
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Micronucleus Tests/*methods
MH  - Mitomycin/toxicity
MH  - Mutagens/*toxicity
MH  - Spindle Apparatus/*drug effects/genetics
MH  - Vinblastine/toxicity
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1093/mutage/10.6.513 [doi]
PST - ppublish
SO  - Mutagenesis. 1995 Nov;10(6):513-6. doi: 10.1093/mutage/10.6.513.