PMID- 8600296
OWN - NLM
STAT- MEDLINE
DCOM- 19960430
LR  - 20071114
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 42
IP  - 5
DP  - 1995 Dec
TI  - Brain-derived neurotrophic factor induces functional expression and phenotypic 
      differentiation of cultured fetal neuropeptide Y-producing neurons.
PG  - 638-47
AB  - A series of studies from our laboratory has established an aggregate culture 
      system of fetal rat brain cells that can serve as a model for studying regulatory 
      processes of the developing neuropeptide Y (NPY)-producing neurons. Using 
      aggregate cultures derived from 17-day-old fetal rat cortex, we addressed these 
      questions: 1) Does brain-derived neurotrophic factor (BDNF) stimulate NPY 
      production, and if so, is stimulation a function of the developmental state of 
      the cultured NPY neuron? 2) Does BDNF induce phenotypic differentiation of NPY 
      neurons? BDNF led to an increase in NPY production and the accumulation of 
      NPY-mRNA in a dose dependent manner. BDNF did not alter the stability of 
      NPY-mRNA, judged by the disappearance rate of NPY-mRNA after blockade of RNA 
      synthesis (estimated t1/2 was 6-8 hr). BDNF stimulation of NPY production was 
      dependent on length of exposure to BDNF and on culture-age. A continuous 8-day 
      exposure to BDNF resulted in a significantly higher level of NPY production than 
      a pulse of 2 days (comparing BDNF exposure on days 0-8 vs. 6-8, or days 8-17 vs. 
      15-17). Moreover, older neurons (age 17 days) produced twice as much NPY as 
      younger (age 8 days) neurons in response to a 2-day pulse of BDNF (50 ng/ml). 
      BDNF was significantly more effective than NT-3 in inducing NPY production, and 
      NGF was ineffective. Immunocytochemical analysis of 8-day NPY neurons revealed 
      that a 2-day pulse of BDNF induced the appearance of an abundance of 
      morphologically well-defined neurons bearing an elaborate network of neurites. 
      This was in contrast to the control-treated NPY neurons, which were 
      morphologically undefined. In summary, the age-dependent effect of BDNF on NPY 
      production is consistent with induction of functional expression, rather than 
      promotion of survival, of cultured NPY neurons. The neurotrophin specificity for 
      stimulation of NPY production, and the lack of effect of BDNF on the stability of 
      NPY-mRNA, implicate the TrkB receptor in mediating transcriptional activation of 
      the NPY gene. Thus, BDNF exerts a dual effect on developing cultured NPY neurons: 
      induction of functional expression, and phenotypic differentiation of immature 
      neurons into mature neurite-bearing neurons.
FAU - Barnea, A
AU  - Barnea A
AD  - Department of Obstetrics and Gynecology, University of Texas Southwestern Medical 
      Center at Dallas 75235-9032, USA.
FAU - Cho, G
AU  - Cho G
FAU - Lu, G
AU  - Lu G
FAU - Mathis, M
AU  - Mathis M
LA  - eng
GR  - NS32207/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Immunohistochemistry
MH  - Iodine Radioisotopes
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Neuropeptide Y/*biosynthesis/genetics
MH  - Neurotrophin 3
MH  - Phenotype
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Transcription, Genetic/drug effects
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1002/jnr.490420506 [doi]
PST - ppublish
SO  - J Neurosci Res. 1995 Dec;42(5):638-47. doi: 10.1002/jnr.490420506.