PMID- 8600926 OWN - NLM STAT- MEDLINE DCOM- 19960503 LR - 20190825 IS - 0886-4470 (Print) IS - 0886-4470 (Linking) VI - 122 IP - 4 DP - 1996 Apr TI - Brain-derived neurotrophic factor and collagen tubulization enhance functional recovery after peripheral nerve transection and repair. PG - 407-13 AB - OBJECTIVE: To investigate the effect of brain-derived neurotrophic factor (BDNF) and collagen tubulization (CT) on the regeneration of transected peripheral nerves. METHODS AND DESIGNS: The left sciatic nerve of 40 Sprague-Dawley rats was transected then repaired using one of four techniques; epineurial coaptation, CT, CT with BDNF delivered by an osmotic pump to the repair site, or CT with BDNF covalently cross-linked to the collagen matrix (CT/linked-BDNF). Sciatic functional indices were measured preoperatively at 10-day intervals for 90 days. Segments of the sciatic nerves proximal and distal to the repair site were harvested at 90 days for histologic and morphometric evaluation. RESULTS: Animals repaired by CT/linked-BDNF (n=10) demonstrated the most favorable functional recovery of all groups, with statistically significant differences seen compared with animals repaired by CT (n=10, P=.05) and epineural coaptation (n=9, P<.001). Animals repaired by CT with BDNF delivered by an osmotic pump (n=8) and CT also showed statistically superior functional recovery compared with those repaired by epineurial coaptation (P=.005 and P=.02, respectively). Nerves repaired by CT/linked-BDNF had the largest mean axon diameters proximal and distal to the repair site. CONCLUSION: Brain-derived neurotrophic factor and CT improve the rate and the degree to which recovery of sciatic function occurs after nerve transection and repair. Animals repaired by CT/BDNF-linked demonstrated the most favorable functional recovery of all groups. Animals whose repair technique included BDNF had the largest mean axon diameters of all groups. FAU - Utley, D S AU - Utley DS AD - Division of Otolaryngology-Head and Neck Surgery, Stanford University Medical Center, Calif., USA. FAU - Lewin, S L AU - Lewin SL FAU - Cheng, E T AU - Cheng ET FAU - Verity, A N AU - Verity AN FAU - Sierra, D AU - Sierra D FAU - Terris, D J AU - Terris DJ LA - eng PT - Comparative Study PT - Journal Article PL - United States TA - Arch Otolaryngol Head Neck Surg JT - Archives of otolaryngology--head & neck surgery JID - 8603209 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Nerve Tissue Proteins) RN - 9007-34-5 (Collagen) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor MH - Collagen/*pharmacology MH - Disease Models, Animal MH - Drug Evaluation, Preclinical MH - Infusion Pumps, Implantable MH - Male MH - Nerve Regeneration/*drug effects MH - Nerve Tissue Proteins/*pharmacology MH - Random Allocation MH - Rats MH - Rats, Sprague-Dawley MH - Sciatic Nerve/*drug effects/*injuries/pathology/surgery MH - Single-Blind Method MH - Suture Techniques EDAT- 1996/04/01 00:00 MHDA- 1996/04/01 00:01 CRDT- 1996/04/01 00:00 PHST- 1996/04/01 00:00 [pubmed] PHST- 1996/04/01 00:01 [medline] PHST- 1996/04/01 00:00 [entrez] AID - 10.1001/archotol.1996.01890160047009 [doi] PST - ppublish SO - Arch Otolaryngol Head Neck Surg. 1996 Apr;122(4):407-13. doi: 10.1001/archotol.1996.01890160047009.