PMID- 8601340
OWN - NLM
STAT- MEDLINE
DCOM- 19960509
LR  - 20190830
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 104
IP  - 6
DP  - 1996 Mar
TI  - Robertsonian polymorphism in the marine gastropod, Nucella lapillus: advances in 
      karyology using rDNA loci and NORs.
PG  - 455-60
AB  - Previous studies of the Robertsonian polymorphism in the Atlantic dog-whelk, 
      Nucella lapillus (2n = 26-36), have been limited by the inability to identify 
      unequivocally individual chromosomes in the karyotype. This species, as with many 
      other marine invertebrates, has proven largely refractory to the standard 
      (mammalian) chromosome-banding techniques. In this study, fluorescence in situ 
      hybridization (FISH) using a rDNA probe was applied to the metaphase chromosomes 
      of the 2n = 26 and 2n = 36 forms of N. lapillus. The results were compared with 
      silver-staining of the nucleolar organizer regions (NORs). The FISH technique was 
      shown to be more sensitive and less intrinsically prone to variation than the 
      silver-staining method. An additional NOR/rDNA locus was observed in the 2n = 36 
      form which, to date, has not been seen in any 2n = 26 population. The 2n = 36 
      karyotype is described for a south-west UK population that differs from that 
      reported previously in the literature. After fission, Robertsonian metacentrics 
      are shown to correspond to at least one subtelocentric product.
FAU - Pascoe, P L
AU  - Pascoe PL
AD  - Plymouth Marine Laboratory, Citadel Hill, Plymouth, Devon, PL1 2PB, UK.
FAU - Patton, S J
AU  - Patton SJ
FAU - Critcher, R
AU  - Critcher R
FAU - Dixon, D R
AU  - Dixon DR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Chromosomes/genetics
MH  - DNA Probes
MH  - DNA, Ribosomal
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Metaphase/physiology
MH  - Nucleolus Organizer Region
MH  - *Polymorphism, Genetic
MH  - Silver Staining
MH  - Snails/*genetics
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1007/BF00352269 [doi]
PST - ppublish
SO  - Chromosoma. 1996 Mar;104(6):455-60. doi: 10.1007/BF00352269.