PMID- 8602587
OWN - NLM
STAT- MEDLINE
DCOM- 19960503
LR  - 20180330
IS  - 0002-9165 (Print)
IS  - 0002-9165 (Linking)
VI  - 63
IP  - 3
DP  - 1996 Mar
TI  - Linoleic acid activates nuclear transcription factor-kappa B (NF-kappa B) and 
      induces NF-kappa B-dependent transcription in cultured endothelial cells.
PG  - 322-8
AB  - High dietary intakes of unsaturated fats may be atherogenic by disrupting normal 
      functions of the vascular endothelium, due in part to the ability of linoleic 
      acid (18:2n-6) to contribute to an increase in cellular oxidative stress and 
      related injurious events. Exposing endothelial cells to 90 micromol linoleic 
      acid/L for 6 h resulted in a significant increase in lipid hydroperoxides that 
      coincided wih an increase in intracellular calcium concentrations. Treatment with 
      this fatty acid caused an initial decrease in glutathione concentrations, which 
      was followed by an increase at later time points. Most importantly, a significant 
      activation of the oxidative stress-sensitive nuclear transcription factor-kappa B 
      (NF-kappa B) was achieved after a 6-h exposure to 18:2n-6, which is the time 
      point at which maximal depletion of cellular glutathione was observed. The fatty 
      acid-mediated NF-kappa B activation was accompanied by induction of NF-kappa 
      B-dependent transcription, as measured by chloramphenicol acetyltransferase (CAT) 
      assay of an NF-kappa B-responsive promoter construct. Pretreatment of endothelial 
      cells with vitamin E and N-acetyl cysteine inhibited the fatty acid-induced 
      activation of NF-kappa B and formation of lipid hydroperoxides. These data 
      suggest that oxidative stress-induced cellular changes are critical early events 
      in fatty acid-mediated endothelial cell dysfunction.
FAU - Hennig, B
AU  - Hennig B
AD  - Department of Nutrition and Food Science, University of Kentucky, Lexington, USA.
FAU - Toborek, M
AU  - Toborek M
FAU - Joshi-Barve, S
AU  - Joshi-Barve S
FAU - Barger, S W
AU  - Barger SW
FAU - Barve, S
AU  - Barve S
FAU - Mattson, M P
AU  - Mattson MP
FAU - McClain, C J
AU  - McClain CJ
LA  - eng
GR  - P42 ES007380/ES/NIEHS NIH HHS/United States
GR  - 1P01 HL36552/HL/NHLBI NIH HHS/United States
GR  - M01 RR02602-08/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Clin Nutr
JT  - The American journal of clinical nutrition
JID - 0376027
RN  - 0 (Linoleic Acids)
RN  - 0 (NF-kappa B)
RN  - 9KJL21T0QJ (Linoleic Acid)
RN  - GAN16C9B8O (Glutathione)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Calcium/metabolism
MH  - Cells, Cultured
MH  - Endothelium, Vascular/drug effects/*metabolism
MH  - Glutathione/metabolism
MH  - Linoleic Acid
MH  - Linoleic Acids/*pharmacology
MH  - Lipid Peroxidation/drug effects
MH  - Molecular Sequence Data
MH  - NF-kappa B/*physiology
MH  - Pulmonary Artery
MH  - Swine
MH  - Transcription, Genetic/*drug effects
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1093/ajcn/63.3.322 [doi]
PST - ppublish
SO  - Am J Clin Nutr. 1996 Mar;63(3):322-8. doi: 10.1093/ajcn/63.3.322.