PMID- 8603798
OWN - NLM
STAT- MEDLINE
DCOM- 19960516
LR  - 20201209
IS  - 0278-6915 (Print)
IS  - 0278-6915 (Linking)
VI  - 34
IP  - 1
DP  - 1996 Jan
TI  - Local lymph node activation in rats after dermal application of the sensitizers 
      2,4-dinitrochlorobenzene and trimellitic anhydride.
PG  - 55-62
AB  - Five rat strains were compared for their performance in the local lymph node 
      assay (LLNA), a promising test system for the identification of the 
      skin-sensitizing potential of chemicals in the mouse. The contract sensitizer 
      2,4-dinitrochlorobenzene (DNCB) and the contact and respiratory sensitizer 
      trimellitic anhydride (TMA) were used as model chemicals and responses in rats 
      were compared with those in BALB/c mice. The chemicals were applied to the dorsum 
      of both ears, once daily for three consecutive days; 2 days (mice) or 3 days 
      (rats) thereafter, proliferating cells were labelled by i.p. injection of BrdU 2 
      hr before the animals were killed. Systemic effects were subsequently assessed by 
      determination of spleen, liver and kidney weights, skin effects by determination 
      of swelling and inflammatory cell infiltration of the ears, and immune effects by 
      determination of weight and proliferative activity of the local lymph nodes 
      (LLN). Following application (x 3) of DNCB or TMA, minor systemic effects were 
      observed, as indicated by slightly elevated spleen and liver weights in a few rat 
      strains and the mice. Skin effects, consisting of increased ear thickness and 
      presence of mononuclear inflammatory cell infiltrates, were observed in all rat 
      strains treated with DNCB or TMA, LLN weights had increased, as had the 
      proliferative activity in these nodes. It was concluded that effects induced by 
      DNCB and TMA in all five rat strains were comparable with those in mice.
FAU - Arts, J H
AU  - Arts JH
AD  - TNO Nutrition and Food Research Institute, Division of Toxicology, Zeist, The 
      Netherlands.
FAU - Droge, S C
AU  - Droge SC
FAU - Bloksma, N
AU  - Bloksma N
FAU - Kuper, C F
AU  - Kuper CF
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Allergens)
RN  - 0 (Antimetabolites)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Irritants)
RN  - 0 (Phthalic Anhydrides)
RN  - 80T61EUU7H (trimellitic anhydride)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Administration, Topical
MH  - Allergens/administration & dosage/*toxicity
MH  - Animals
MH  - Antimetabolites
MH  - Bromodeoxyuridine
MH  - Cell Division/drug effects
MH  - Dinitrochlorobenzene/administration & dosage/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Ear, External/drug effects
MH  - Female
MH  - Irritants/administration & dosage/*toxicity
MH  - Kidney/drug effects
MH  - Liver/drug effects
MH  - Lymph Nodes/cytology/*drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Organ Size/drug effects
MH  - Phthalic Anhydrides/administration & dosage/*toxicity
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred F344
MH  - Rats, Inbred Strains
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Skin/drug effects
MH  - Spleen/drug effects
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 0278691595000895 [pii]
AID - 10.1016/0278-6915(95)00089-5 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 1996 Jan;34(1):55-62. doi: 10.1016/0278-6915(95)00089-5.