PMID- 8603934
OWN - NLM
STAT- MEDLINE
DCOM- 19960514
LR  - 20190920
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 15
IP  - 6
DP  - 1995 Nov-Dec
TI  - Evaluation of lymphocyte proliferation by immunohistochemistry in the local lymph 
      node assay.
PG  - 465-75
AB  - A modified version of the local lymph node assay (LLNA) is presented, using 
      bromodeoxyuridine to label proliferating lymphocytes. Cell counting is done on 
      mid-sagittal sections of individual nodes under light microscopy. Two irritants 
      (sodium lauryl sulfate and salicylic acid), four allergens of various sensitizing 
      potential (potassium dichromate, 4-chloroaniline, neomycin sulfate and nickel 
      sulfate) and one chemical of unknown sensitizing potential (ethyl 
      3-aminobenzoate) were tested either in the short protocol using three daily 
      topical applications and/or in the long protocol with a pre-exposure step under 
      an occluded patch. A weak T cell proliferation was noted with both irritants in 
      the short protocol, but not in the long protocol. Potassium dichromate induced a 
      strong proliferative response in the short protocol. A lesser sensitizing 
      potential was detected for 4-chloroaniline, ethyl 3-aminobenzoate and neomycin 
      sulfate but only in the long protocol. Nickel sulfate was negative in both 
      protocols. The long protocol was the most valuable for weak or moderate 
      sensitizers. Histological examination of nodes ruled out intercurrent processes. 
      The present procedure offers several advantages. The use of a non-isotopic marker 
      enables this test to be run in a routine safety assessment department and allows 
      the preparation of permanent slides. An increased specificity is obtained by 
      restricting cell counting to the paracortex. Moreover, the collection of 
      individual data permits statistical analysis of the results. This method is 
      sensitive and reproducible and may be viewed as a useful adjunct to the LLNA.
FAU - Boussiquet-Leroux, C
AU  - Boussiquet-Leroux C
AD  - Laboratoires Merck Sharp & Dohme-Chibret, Department of Safety Assessment, 
      Research Center, Riom, France.
FAU - Durand-Cavagna, G
AU  - Durand-Cavagna G
FAU - Herlin, K
AU  - Herlin K
FAU - Holder, D
AU  - Holder D
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - T4423S18FM (Potassium Dichromate)
RN  - U3RSY48JW5 (Benzocaine)
SB  - IM
MH  - Animals
MH  - Benzocaine/toxicity
MH  - Dermatitis, Irritant/immunology/pathology
MH  - Female
MH  - Immunohistochemistry
MH  - Lymph Nodes/*immunology
MH  - *Lymphocyte Activation/drug effects
MH  - Lymphocyte Count
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Organ Size/drug effects
MH  - Potassium Dichromate/toxicity
MH  - Sodium Dodecyl Sulfate/toxicity
MH  - T-Lymphocytes/immunology
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1002/jat.2550150608 [doi]
PST - ppublish
SO  - J Appl Toxicol. 1995 Nov-Dec;15(6):465-75. doi: 10.1002/jat.2550150608.