PMID- 8604018
OWN - NLM
STAT- MEDLINE
DCOM- 19960515
LR  - 20191210
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 59
IP  - 3
DP  - 1996 Mar
TI  - Activation of alveolar macrophage TNF and MCP-1 expression in vivo by a synthetic 
      peptide of C-reactive protein.
PG  - 397-402
AB  - Administration of multilamellar vesicles (MLV) encapsulating a synthetic peptide 
      (RS-83277) derived from human C-reactive protein (CRP) augments anti-tumor 
      activity of murine alveolar macrophages and reduces established pulmonary 
      metastases of experimental tumors. To explore mechanisms involved in these 
      phenomena, we investigated cytokine and integrin (CDllb) expression of 
      bronchoalveolar lavage (BAL)-derived alveolar macrophages in control (blank MLV) 
      and RS-83277-MLV-treated C57BI mice. Alveolar macrophage production of tumor 
      necrosis factor alpha (TNF-alpha) and monocyte chemoattractant bioactivity 
      increased at 48 h after treatment with RS-83277-MLV but not control MLV. 
      Chemoattractant activity was neutralized by antibody to monocyte chemoattractant 
      protein-1 (MCP-1), but not irrelevant immunoglobulin G(IgG). Changes were 
      reflected by augmented TNF-alpha and MCP-1 mRNA levels in pulmonary tissue and 
      enhanced CD11b expression on mononuclear leukocytes derived from total lung 
      tissue, but not on BAL-derived alveolar macrophages. Results suggest that 
      RS-83277-MLV treatment is associated with activation of alveolar macrophage 
      TNF-alpha and MCP-1 production and up-regulation of adhesion molecules on 
      pulmonary mononuclear leukocytes but not on alveolar macrophages.
FAU - Barna, B P
AU  - Barna BP
AD  - Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio, USA.
FAU - Thomassen, M J
AU  - Thomassen MJ
FAU - Zhou, P
AU  - Zhou P
FAU - Pettay, J
AU  - Pettay J
FAU - Singh-Burgess, S
AU  - Singh-Burgess S
FAU - Deodhar, S D
AU  - Deodhar SD
LA  - eng
GR  - CA49950/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Immunologic Factors)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (RS 83277)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - C-Reactive Protein/chemistry/*pharmacology
MH  - Chemokine CCL2/*metabolism
MH  - Chemokines/genetics
MH  - Cytokines/genetics
MH  - Gene Expression
MH  - Immunologic Factors/pharmacology
MH  - Intercellular Signaling Peptides and Proteins
MH  - *Macrophage Activation
MH  - Macrophages, Alveolar/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Peptide Fragments/pharmacology
MH  - Peptides/pharmacology
MH  - RNA, Messenger/genetics
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1002/jlb.59.3.397 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1996 Mar;59(3):397-402. doi: 10.1002/jlb.59.3.397.