PMID- 8604312
OWN - NLM
STAT- MEDLINE
DCOM- 19960515
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 24
IP  - 4
DP  - 1996 Feb 15
TI  - A novel type of retinoic acid response element in the second intron of the mouse 
      H2Kb gene is activated by the RAR/RXR heterodimer.
PG  - 694-701
AB  - We have identified and characterized a novel retinoic acid (RA) response element 
      (Hi-RARE) in the second intron of the mouse major histocompatibility H2Kb gene. 
      The Hi-RARE sequence is conserved in all mouse classical and Q class 1 genes, in 
      MHC class 1 genes of the rat, Rhesus macaque, cat and in the vast majority of 
      human classical and non-classical class 1 genes. The Hi-RARE sequence lies within 
      a regulatory element responsible for constitutive expression of a 5' enhancerless 
      H2Kb gene in the Ltk-fibroblasts. Hi-RARE consists of two inverted palindromic 
      RARE consensus sites (5'-PuGGTCA-3') separated by an 8 nt spacer. Mutational 
      analysis revealed that both inverted palindromic hexanucleotide motifs are 
      indispensable functional sites for the 9-cis RA response. The Hi-RARE sequence 
      confers 9-cis RA inducibility to a heterologous promoter. The inducibility is 
      further augmented in embryonal carcinoma cells by the expression of recombinant 
      retinoic acid receptors (PARs) and the retinoid X receptors (RXRs). In vitro, the 
      recombinant RAR/RXR heterodimer creates DNA-protein complex with the Hi-RARE 
      sequence. Treatment of P19 embryonal carcinoma cells with 9C-RA induces the 
      Hi-RARE binding activity of nuclear proteins that proved to be RAR (or 
      RAR-Like)/RXR heterodimer. Thus the Hi-RARE represents a new type of RA response 
      element with a role in the modulation of the expression of MHC class 1 family 
      genes.
FAU - Jansa, P
AU  - Jansa P
AD  - Laboratory of Mammalian Molecular Genetics, Institute of Molecular Genetics, 
      Academy of Sciences of Czech Republic, Prague.
FAU - Forejt, J
AU  - Forejt J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Conserved Sequence
MH  - Introns/genetics
MH  - Major Histocompatibility Complex/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Receptors, Retinoic Acid/*genetics/metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Retinoid X Receptors
MH  - Sequence Alignment
MH  - Transcription Factors/*genetics/metabolism
MH  - Tretinoin/*metabolism
PMC - PMC145677
EDAT- 1996/02/15 00:00
MHDA- 1996/02/15 00:01
PMCR- 1996/02/15
CRDT- 1996/02/15 00:00
PHST- 1996/02/15 00:00 [pubmed]
PHST- 1996/02/15 00:01 [medline]
PHST- 1996/02/15 00:00 [entrez]
PHST- 1996/02/15 00:00 [pmc-release]
AID - 5b0246 [pii]
AID - 10.1093/nar/24.4.694 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1996 Feb 15;24(4):694-701. doi: 10.1093/nar/24.4.694.