PMID- 8604688
OWN - NLM
STAT- MEDLINE
DCOM- 19960515
LR  - 20190512
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 105
IP  - 4
DP  - 1996 Apr
TI  - Karyotype correlates with peripheral blood morphology and immunophenotype in 
      chronic lymphocytic leukemia.
PG  - 458-67
AB  - Chronic lymphocytic leukemia (CLL) is recognized as a distinct entity. However, 
      morphologic and immunophenotypic heterogeneity exist. Twenty-six patients with 
      CLL were studied to investigate whether an association exists among peripheral 
      blood karyotype, morphology and immunophenotype. Clonal cytogenetic abnormalities 
      were detected in 14 patients (53%), using conventional karyotyping techniques in 
      addition to fluorescence in situ hybridization (FISH) for chromosome 12. By FAB 
      guidelines, 7 of the 8 patients (88%) with trisomy 12 had mixed cell morphology 
      compared to only 3 of 18 (17%) without trisomy 12 (P = .004). One patient (12%) 
      with trisomy 12 had lymphocyte morphology typical for CLL. Six of the eight (75%) 
      with trisomy 12 had atypical immunophenotype including one or more of the 
      following: strong CD20 expression, strong surface light chain expression, or 
      absence of CD23 expression. Only 2 of the 18 patients (11%) without trisomy 12 
      had atypical immunophenotype (P = .005). None of the three patients with clonal 
      structural abnormalities of chromosome 13q14 had mixed cell morphology or 
      atypical immunophenotype. One of the 12 patients (8%) without clonal cytogenetic 
      abnormalities had mixed cell morphology and one had atypical immunophenotype. 
      This study suggests that a correlation exists among karyotype, morphology, and 
      immunophenotype in CLL, and that CLL subgroups can be identified based on 
      laboratory parameters. Although normal karyotypes or clonal structural 
      abnormalities of 13q14 are associated with morphology and immunophenotype 
      considered typical for CLL, trisomy 12 is associated with mixed cell morphology 
      and atypical immunophenotype. These findings may have implications for evaluating 
      variation in both disease course and response to emerging therapies.
FAU - Finn, W G
AU  - Finn WG
AD  - Northwestern University Medical School, Chicago, Illinois 60611, USA.
FAU - Thangavelu, M
AU  - Thangavelu M
FAU - Yelavarthi, K K
AU  - Yelavarthi KK
FAU - Goolsby, C L
AU  - Goolsby CL
FAU - Tallman, M S
AU  - Tallman MS
FAU - Traynor, A
AU  - Traynor A
FAU - Peterson, L C
AU  - Peterson LC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Aged
MH  - Antigens, CD/analysis
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - Karyotyping
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/blood/classification/immunology/*pathology
MH  - Lymphocytes/*immunology/*pathology
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Trisomy
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1093/ajcp/105.4.458 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 1996 Apr;105(4):458-67. doi: 10.1093/ajcp/105.4.458.