PMID- 8605113
OWN - NLM
STAT- MEDLINE
DCOM- 19960521
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 14
IP  - 4
DP  - 1995 Dec
TI  - Identification of multiple chromosome 12 abnormalities in human testicular germ 
      cell tumors by two-color fluorescence in situ hybridization (FISH).
PG  - 252-8
AB  - The distribution of segments of the short and long arms of chromosome 12 was 
      distinguished by two-color fluorescence in situ hybridization (FISH) in 27 
      cytogenetically abnormal testicular germ cell tumors (TGCTs). A 12p-specific 
      probe was developed by chromosomal microdissection and sequence-independent 
      polymerase chain reaction (PCR) amplification and was combined with a 
      commercially available whole-chromosome 12 painting probe. The TGCTs included 
      both i(12p)-positive and i(12p)-negative primary tumors and lymph node metastases 
      from patients in clinical stage I or stage II who were not previously treated 
      with chemotherapy. Rearrangements of the short arm of chromosome 12 and 
      overrepresentation of 12p DNA sequences were found in all cases. In addition, 
      cryptic rearrangements of 12p were found in 39% (7/18) of the i(12p)-positive 
      tumors and in 78% (7/9) of the i(12p)-negative tumors. Only 7% (2/27) of all 
      tumors had cryptic rearrangements of 12q.
FAU - Smolarek, T A
AU  - Smolarek TA
AD  - Department of Medical and Molecular Genetics, Indiana University School of 
      Medicine, Indianapolis, USA.
FAU - Blough, R I
AU  - Blough RI
FAU - Foster, R S
AU  - Foster RS
FAU - Ulbright, T M
AU  - Ulbright TM
FAU - Palmer, C G
AU  - Palmer CG
FAU - Heerema, N A
AU  - Heerema NA
LA  - eng
GR  - R35 CA39844/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - *Chromosomes, Human, Pair 12
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lymph Nodes/pathology
MH  - Lymphatic Metastasis/pathology
MH  - Male
MH  - Neoplasms, Germ Cell and Embryonal/*genetics/pathology
MH  - Testicular Neoplasms/*genetics/pathology
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1002/gcc.2870140403 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1995 Dec;14(4):252-8. doi: 10.1002/gcc.2870140403.