PMID- 8605732
OWN - NLM
STAT- MEDLINE
DCOM- 19960521
LR  - 20191024
IS  - 0262-0898 (Print)
IS  - 0262-0898 (Linking)
VI  - 14
IP  - 2
DP  - 1996 Mar
TI  - Invasion-inhibiting factor 2-albumin conjugate inhibits invasion and spontaneous 
      metastasis of MKL-4 human breast cancer cells transplanted into female nude mice.
PG  - 87-94
AB  - Invasion-inhibiting factor 2 (IIF-2) and its albumin conjugate have been reported 
      to inhibit spontaneous metastasis of highly metastatic cancer cells with no 
      effect on primary tumor growth. To confirm the inhibitory effects of the IIF-2 
      conjugate on tumor invasion and spontaneous metastasis, we administered the 
      conjugate intra-peritoneally (i.p.) to female nude mice bearing transplanted 
      tumors with MKL-4 cells, which are MCF-7 human breast cancer cells cotransfected 
      with fibroblast growth factor 4 and lacZ. Neither 10 nor 20 mg/kg doses of the 
      conjugate caused any inhibition of primary tumor growth, but 20 mg/kg 
      significantly inhibited tumor invasion and spontaneous metastasis. Tumor invasion 
      was measured by a novel computer-assisted image analysis. Spontaneous microscopic 
      metastases into lymph nodes and distant organs were measured by whole organ 
      staining for beta-galactosidase activity and observed with a dissecting 
      microscope. The dose of 10 mg/kg significantly inhibited tumor invasion but not 
      metastasis. Interestingly, the number of factor VIII-positive microvessels in the 
      tumors was not reduced by treatment at either dose level. These findings suggest 
      that the anti-invasive effect of the IIF-2 conjugate may reduce both lymphatic 
      and hematogenous metastases in this MKL-4 metastasis model without affecting 
      angiogenesis.
FAU - Kurebayashi, J
AU  - Kurebayashi J
AD  - Department of Endocrine Surgery, Kawasaki Medical School, Okayama, Japan.
FAU - Kurosumi, M
AU  - Kurosumi M
FAU - Isoai, A
AU  - Isoai A
FAU - Dickson, R B
AU  - Dickson RB
FAU - Sonoo, H
AU  - Sonoo H
LA  - eng
GR  - 1 P30CA51008/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Clin Exp Metastasis
JT  - Clinical & experimental metastasis
JID - 8409970
RN  - 0 (Angiogenesis Inhibitors)
RN  - 0 (Proteins)
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Angiogenesis Inhibitors
MH  - Animals
MH  - Breast Neoplasms/blood supply/*pathology
MH  - Cell Division
MH  - Humans
MH  - Lymphatic Metastasis
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - Proteins/*administration & dosage
MH  - Serum Albumin/chemistry
MH  - Tumor Cells, Cultured
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1007/BF00121205 [doi]
PST - ppublish
SO  - Clin Exp Metastasis. 1996 Mar;14(2):87-94. doi: 10.1007/BF00121205.