PMID- 8605919
OWN - NLM
STAT- MEDLINE
DCOM- 19960520
LR  - 20061115
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 26
IP  - 3
DP  - 1996 Mar
TI  - Immunization with glycosylated Kb-binding peptides generates 
      carbohydrate-specific, unrestricted cytotoxic T cells.
PG  - 544-51
AB  - Cytotoxic T cells (CTL) recognize target proteins as short peptides presented by 
      major histocompatibility complex (MHC) class I restriction elements. However, 
      there is also evidence for peptide-independent T cell receptor (TCR) recognition 
      of target proteins and non-protein structures. How such T cell responses are 
      generated is presently unclear. We generated carbohydrate (CHO)-specific, 
      MHC-unrestricted CTL responses by coupling di- and trisaccharides to Kb- or 
      Db-binding peptides for direct immunization in mice. Four peptides and three CHO 
      have been analyzed with the CHO either in terminal or central position on the 
      carrier peptide. With two of these glycopeptides, with galabiose (Gal alpha 
      1-4Gal; Gal2) bound to a homocysteine (via an ethylene spacer arm) in position 4 
      or 6 in a vesicular stomatitis virus nucleoprotein-derived peptide (RGYVYQGL 
      binding to Kb), CTL were generated which preferentially killed target cells 
      treated with glycopeptide compared to those treated with the core peptide. 
      Polyclonal CTL were also found to kill target cells expressing the same Gal2 
      epitope in a glycolipid. By fractionation of CTL, preliminary data indicate that 
      glycopeptide-specific Kb-restricted CTL and unrestricted CHO-specific CTL belong 
      to different T cell populations with regard to TCR expression. The results 
      demonstrate that hapten-specific unrestricted CTL responses can be generated with 
      MHC class I-binding carrier peptides. Different models that might explain the 
      generation of such responses are discussed.
FAU - Abdel-Motal, U M
AU  - Abdel-Motal UM
AD  - Department of Medical Biochemistry and Microbiology, University of Gothenburg, 
      Sweden.
FAU - Berg, L
AU  - Berg L
FAU - Rosen, A
AU  - Rosen A
FAU - Bengtsson, M
AU  - Bengtsson M
FAU - Thorpe, C J
AU  - Thorpe CJ
FAU - Kihlberg, J
AU  - Kihlberg J
FAU - Dahmen, J
AU  - Dahmen J
FAU - Magnusson, G
AU  - Magnusson G
FAU - Karlsson, K A
AU  - Karlsson KA
FAU - Jondal, M
AU  - Jondal M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Carbohydrates)
RN  - 0 (Epitopes)
RN  - 0 (Glycopeptides)
RN  - 0 (H-2 Antigens)
RN  - 0 (H-2Kb protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carbohydrate Sequence
MH  - Carbohydrates/*immunology
MH  - Epitopes/*immunology
MH  - Glycopeptides/*immunology/metabolism/pharmacology
MH  - Glycosylation
MH  - H-2 Antigens/*immunology/*metabolism
MH  - *Immunization
MH  - *Lymphocyte Activation/drug effects
MH  - Mice
MH  - Molecular Sequence Data
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1002/eji.1830260307 [doi]
PST - ppublish
SO  - Eur J Immunol. 1996 Mar;26(3):544-51. doi: 10.1002/eji.1830260307.