PMID- 8605982
OWN - NLM
STAT- MEDLINE
DCOM- 19960517
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 382
IP  - 3
DP  - 1996 Mar 18
TI  - Anti-head and anti-tail antibodies against distinct epitopes in the catalytic 
      subunit of protein kinase A. Use in the study of the kinase splitting membranal 
      proteinase KSMP.
PG  - 265-70
AB  - Protein kinases share a considerable sequence homology in their catalytic core 
      (residues 40-300 in PKA). Each core is flanked by "head" and "tail" segments at 
      its amino- and carboxy-termini, which are different in the various kinases. These 
      end segments may play an important role in creating the preferential affinity of 
      each kinase for its physiological substrates or regulatory ligands. Here we 
      describe three anti-peptide antibodies (alpha P-1, alpha P-2, and alpha P-3) that 
      specifically recognize the head and tail segments of the catalytic subunit (C) of 
      PKA. (i) alpha P-1 (against 6A-K23) react with C when denatured but not when in 
      its native structure; (ii) alpha P-2 (against 319K-I335), bind to the site in C 
      cleaved by the kinase splitting membranal proteinase (KSMP) and inhibit this 
      cleavage of C; (iii) alpha P-3 (against 338S-F350) react with C but not with the 
      KSMP cleavage product C', useful for detecting a KSMP-like activity in different 
      tissues and subcellular loci. The combined use of the antibodies described here 
      provides a strict definition of C, and thus a high degree of fidelity in its 
      biorecognition.
FAU - Chestukhin, A
AU  - Chestukhin A
AD  - Department of Biological Regulation, The Weizmann Institute of Science, Rehovot, 
      Israel.
FAU - Litovchick, L
AU  - Litovchick L
FAU - Batkin, M
AU  - Batkin M
FAU - Shaltiel, S
AU  - Shaltiel S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Antibodies)
RN  - 0 (Epitopes)
RN  - 0 (Peptides)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.99.- (kinase-splitting membranal proteinase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antibodies
MH  - Antibody Specificity
MH  - Catalysis
MH  - Cattle
MH  - Cyclic AMP-Dependent Protein Kinases/*chemistry/genetics/metabolism
MH  - Endopeptidases/*metabolism
MH  - Epitopes/*analysis
MH  - Molecular Sequence Data
MH  - Peptides/chemical synthesis/immunology
MH  - Protein Conformation
MH  - Protein Denaturation
MH  - Sequence Deletion
MH  - Substrate Specificity
EDAT- 1996/03/18 00:00
MHDA- 1996/03/18 00:01
CRDT- 1996/03/18 00:00
PHST- 1996/03/18 00:00 [pubmed]
PHST- 1996/03/18 00:01 [medline]
PHST- 1996/03/18 00:00 [entrez]
AID - 0014-5793(96)00178-0 [pii]
AID - 10.1016/0014-5793(96)00178-0 [doi]
PST - ppublish
SO  - FEBS Lett. 1996 Mar 18;382(3):265-70. doi: 10.1016/0014-5793(96)00178-0.