PMID- 8606483
OWN - NLM
STAT- MEDLINE
DCOM- 19960522
LR  - 20061115
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 74
IP  - 4
DP  - 1996 Apr
TI  - In situ detection of frequent and active infection of human cytomegalovirus in 
      inflammatory abdominal aortic aneurysms: possible pathogenic role in sustained 
      chronic inflammatory reaction.
PG  - 723-36
AB  - Inflammatory abdominal aortic aneurysm (IAAA) is histopathologically 
      characterized by extensive adventitial fibrosis, mononuclear cell infiltration 
      with lymph follicle formation, and severe atheromatous changes in the aneurysmal 
      wall. We previously reported a frequent prevalence and immediate early gene 
      expression of human cytomegalovirus (CMV) in IAAA by solution-phase PCR and 
      reverse transcription PCR, respectively, and suggested that this virus might play 
      a role in chronic inflammatory reaction in IAAA. To evaluate the pathogenic role 
      of CMV infection, the frequency and distribution of CMV infected cells in IAAA 
      were examined by in situ PCR, and compared with those in atherosclerotic 
      aneurysms (AA) and control cases with minimal atherosclerotic changes. Human 
      leukocyte antigen (HLA)-DR was simultaneously evaluated as a marker for immune 
      response related to CMV infection. Immediate early gene expression was also 
      detected by reverse transcription PCR and in situ hybridization, to certify 
      whether the CMV infection in IAAA is active or latent. In the fibrously thickened 
      adventitia of IAAA, CMV infected cells and HLA-DR-positive cells were more 
      frequently encountered than in that of AA and control cases (p < 0.01). CMV 
      infected cells were largely identified as macrophages, fibroblasts, endothelial 
      cells, and lymphocytes. The expression of CMV immediate early mRNA, which 
      suggests an active infection inducing active inflammatory reaction, was detected 
      in most of the macrophages, endothelial cells, and fibroblasts. Our results 
      strongly suggest that frequent and active infection of CMV in IAAA plays a 
      significant role in the induction and acceleration of chronic inflammatory 
      reaction in aortas of IAAA.
FAU - Yonemitsu, Y
AU  - Yonemitsu Y
AD  - Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
FAU - Nakagawa, K
AU  - Nakagawa K
FAU - Tanaka, S
AU  - Tanaka S
FAU - Mori, R
AU  - Mori R
FAU - Sugimachi, K
AU  - Sugimachi K
FAU - Sueishi, K
AU  - Sueishi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (DNA, Viral)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Aortic Aneurysm, Abdominal/*complications
MH  - Base Sequence
MH  - Chronic Disease
MH  - Cytomegalovirus/genetics
MH  - Cytomegalovirus Infections/*complications/diagnosis
MH  - DNA, Viral/analysis
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - In Situ Hybridization
MH  - Inflammation/*etiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Rabbits
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1996 Apr;74(4):723-36.