PMID- 8606484
OWN - NLM
STAT- MEDLINE
DCOM- 19960522
LR  - 20061115
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 74
IP  - 4
DP  - 1996 Apr
TI  - Monomeric immunoglobulin A protects glomerulus against polymeric immunoglobulin A 
      immune complex in experimental immunoglobulin A nephropathy.
PG  - 737-46
AB  - Polymeric (p) IgA constitutes 13% of total serum IgA, whereas monomeric (m) IgA 
      represents the other 87%. pIgA tends to form complexes in the circulation that 
      eventually localize in the glomerulus. mIgA is a nonprecipitable antibody. When 
      complexed with an antigen in vivo, the circulating mIgA immune complex (IC) thus 
      formed does not deposit in the glomerulus. The purpose of the present study was 
      to evaluate the influence of mIgA on the formation of pIgA-lC and subsequent 
      glomerular deposition of the IC in an experimental model of IgA nephropathy. The 
      influence of mIgA anti-dinitrophenyl (DNP) on pIgA anti-DNP/DNP-conjugated BSA 
      (BSA-DNP) IC formation was assessed by double diffusion test and competition PAGE 
      in combination with autoradiography. Only the BSA-DNP, a model antigen of 
      relatively low molecular mass, was radiolabeled and monitored throughout the 
      latter experiment. An analysis of clearance kinetics (1 and 6 hours) of the 
      antigen using 4-week-old female BALB/c mice, and a series of renal studies were 
      performed after intravenous injection with a single dose of combined pIgA 
      anti-DNP, BSA-DNP, and mIgA anti-DNP. As demonstrated by the double diffusion 
      test, addition of mIgA anti-DNP resulted in suppression of the precipitating 
      reaction of pIgA anti-DNP and BSA-DNP. This effect was confirmed by PAGE for size 
      determination of the BSA-DNP which had been complexed with either pIgA anti-DNP 
      or a mixture of pIgA anti-DNP and mIgA anti-DNP. The clearance kinetics studies 
      showed that the elimination of BSA-DNP injected with pIgA anti-DNP was prolonged 
      in the presence of mIgA anti-DNP in a partially dose-dependent manner. The 
      experimental mice receiving pIgA anti-DNP, BSA-DNP, and mIgA anti-DNP showed less 
      hematuria (p < 0.005) than mice receiving pIgA anti-DNP and BSA-DNP (positive 
      control) when examined 1 hour after injection. Immunofluorescence study of the 
      renal tissue of mice receiving pIgA anti-DNP, BSA-DNP, and mIgA anti-DNP showed a 
      suppressed glomerular localization of IgA and third component of complement, as 
      compared with those injected with pIgA anti-DNP and BSA-DNP alone. Similarly, a 
      significant decrease of glomerular BSA-DNP deposits was observed in mice 
      receiving pIgA anti-DNP, BSA-DNP, and mIgA anti-DNP compared with those receiving 
      pIgA anti-DNP and BSA-DNP alone, as demonstrated by light microscopic 
      autoradiography. These findings indicate that a high dose of specific mIgA was 
      capable of modulating glomerular deposition of the pIgA-IC in this animal model 
      of IgAN.
FAU - Chen, A
AU  - Chen A
AD  - Department of Pathology, Tri-Service General Hospital, National Defense Medical 
      Center, Taipei, Taiwan, Republic of China.
FAU - Sheu, L F
AU  - Sheu LF
FAU - Ding, S L
AU  - Ding SL
FAU - Lee, W H
AU  - Lee WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Immunoglobulin A)
RN  - 0 (polymeric IgA)
SB  - IM
MH  - Animals
MH  - Antigen-Antibody Complex/*metabolism
MH  - Female
MH  - Glomerulonephritis, IGA/immunology/pathology/*prevention & control
MH  - Immunoglobulin A/*metabolism/*therapeutic use
MH  - Kidney Glomerulus/*immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1996 Apr;74(4):737-46.