PMID- 8607140
OWN - NLM
STAT- MEDLINE
DCOM- 19960517
LR  - 20220318
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 137
IP  - 1
DP  - 1996 Mar
TI  - Mono-(2-ethylhexyl) phthalate rapidly alters both Sertoli cell vimentin filaments 
      and germ cell apoptosis in young rat testes.
PG  - 42-50
AB  - Mono-(2-ethylhexyl) phthalate (MEHP) is a widely studied Sertoli cell toxicant. 
      Here we describe alterations in Sertoli cell vimentin filament distribution and 
      the incidence of testicular germ cell apoptosis in young (28-day-old) Fischer 
      rats that were treated with MEHP (2 microgram/kg, po) and killed 0, 3, 6, or 12 
      hr after exposure. A collapse in vimentin filaments was observed 3 hr after MEHP 
      exposure without accompanying changes in the pattern of Sertoli cell tubulin or 
      actin. A progressive increase in the perinuclear condensation of the vimentin 
      filaments was observed from 6 to 12 hr after exposure. To evaluate the 
      consequences of these Sertoli cell changes on germ cells, the role of apoptosis 
      in MEHP-induced testicular toxicity was examined. DNA isolated from testis of 
      rats 6 and 12 hr after MEHP exposure showed a marked increase in 
      low-molecular-weight DNA resulting from internucleosomal cleavage. In addition, 
      DNA fragmentation visualized in frozen testis cross sections by terminal 
      deoxynucleotidyl transferase-mediated digoxigenin-dUTP nick end label (TUNEL) 
      staining demonstrated a progressive increase in germ cell apoptosis from 6 to 12 
      hr after MEHP exposure. However, 3 hr after MEHP exposure, the incidence of 
      TUNEL-positive germ cells was significantly decreased compared to that seen in 
      controls. Taken together, the early collapse in Sertoli cell vimentin filaments 
      and the concurrent decrease in germ cell apoptosis suggests that MEHP engenders 
      Sertoli cell dysfunction resulting in the disruption of the physiological 
      mechanism of germ cell apoptosis.
FAU - Richburg, J H
AU  - Richburg JH
AD  - Department of Pathology and Laboratory Medicine, Brown University, Providence, 
      Rhode Island 02912, USA.
FAU - Boekelheide, K
AU  - Boekelheide K
LA  - eng
GR  - R01 ES05033/ES/NIEHS NIH HHS/United States
GR  - T32 ES07272/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Vimentin)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
RN  - FU2EWB60RT (mono-(2-ethylhexyl)phthalate)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Count
MH  - Diethylhexyl Phthalate/*analogs & derivatives/toxicity
MH  - Immunohistochemistry
MH  - Intermediate Filaments/chemistry/*drug effects/ultrastructure
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - Sertoli Cells/*drug effects/ultrastructure
MH  - Spermatozoa/cytology/*drug effects
MH  - Staining and Labeling
MH  - Testis/drug effects/ultrastructure
MH  - Vimentin/*analysis
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - S0041-008X(96)90055-1 [pii]
AID - 10.1006/taap.1996.0055 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 1996 Mar;137(1):42-50. doi: 10.1006/taap.1996.0055.