PMID- 8608736
OWN - NLM
STAT- MEDLINE
DCOM- 19960528
LR  - 20071114
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 21
IP  - 4
DP  - 1995 Dec 1
TI  - Reutilization of previously hybridized slides for fluorescence in situ 
      hybridization.
PG  - 378-81
AB  - Application of fluorescence in situ hybridization (FISH) to clinical material is 
      sometimes limited by sample size. In addition, heterogeneity among slides 
      prepared from a single sample may lead to variation in FISH analyses. 
      Reutilization of material for repeated FISH analyses would help to alleviate 
      these problems. We have developed a simple procedure for repeated FISH analyses 
      with directly conjugated probes. Previously hybridized probes are removed by 
      incubation in denaturing solution, and slides can then be rehybridized without 
      residual signals remaining. Several cycles of this procedure allow a full 
      complement of chromosomal loci to be analyzed on the same population of cells. 
      Advantages of this protocol include gaining more cytogenetic information from 
      small samples and eliminating the problem of intratumor variability.
FAU - Epstein, L
AU  - Epstein L
AD  - Department of Laboratory Medicine, University of California, San Francisco 
      94143-0808, USA.
FAU - DeVries, S
AU  - DeVries S
FAU - Waldman, F M
AU  - Waldman FM
LA  - eng
GR  - CA44768/CA/NCI NIH HHS/United States
GR  - CA47537/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Centromere/genetics
MH  - Chromosomes/genetics
MH  - DNA Probes
MH  - DNA, Satellite/analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukocytes, Mononuclear/cytology
MH  - Microscopy, Fluorescence
MH  - Nucleic Acid Hybridization
MH  - Specimen Handling
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1002/cyto.990210410 [doi]
PST - ppublish
SO  - Cytometry. 1995 Dec 1;21(4):378-81. doi: 10.1002/cyto.990210410.