PMID- 8609722
OWN - NLM
STAT- MEDLINE
DCOM- 19960529
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 9
IP  - 12
DP  - 1995 Dec
TI  - Establishment of the novel B acute lymphoblastic leukemia (FAB L3) cell line 
      KHM-10B with a 13q34 abnormality and constitutive expression of c-myc and max 
      during cell cycle.
PG  - 2093-9
AB  - We established and characterized a new acute lymphoblastic leukemia (ALL-L3 
      according to FAB classification, or Burkitt's type) cell line, KHM-10B. The 
      morphology of the patient's lymphoblasts and KHM-10B cells corresponded to that 
      of ALL-L3 cells. The cells were positive for HLA-DR, CD19 and surface 
      immunoglobulin (mu, lambda). Southern blot analysis revealed that the fresh 
      lymphoblasts and KHM-10B shared the same immunoglobulin gene rearrangement. 
      Conventional cytogenetic analysis of fresh lymphoblasts from the patient and 
      KHM-10B cells revealed the 13q34 abnormality, the second most common additional 
      abnormality in Burkitt's lymphoma, but no detectable 8q24 involvement. 
      Rearrangement of the c-myc oncogene was not detected by Southern blot analysis. 
      However, a fluorescence in situ hybridization (FISH) assay identified a 
      t(8;22)(q24;q11). The KHM-10B cells were arrested at S phase with hydroxyurea and 
      thymidine, and the synchronized cells progressed through the cell cycle in 
      drug-free medium. The expression of c-myc and max was observed throughout the 
      cell cycle, as was found in the Burkitt's lymphoma cell in Raji. Our findings 
      indicate that FISH analysis is of diagnostic value in detecting obscure 
      chromosomal translocations and that max, as well as c-myc, is expressed 
      constitutively in ALL-L3 and Burkitt's lymphoma cell lines.
FAU - Sonoki, T
AU  - Sonoki T
AD  - Second Department of Internal Medicine, Kumamoto University School of Medicine, 
      Japan.
FAU - Matsuzaki, H
AU  - Matsuzaki H
FAU - Miyamoto, K
AU  - Miyamoto K
FAU - Taniwaki, M
AU  - Taniwaki M
FAU - Yoshino, T
AU  - Yoshino T
FAU - Hata, H
AU  - Hata H
FAU - Yoshida, M
AU  - Yoshida M
FAU - Matsuno, F
AU  - Matsuno F
FAU - Nagasaki, A
AU  - Nagasaki A
FAU - Kuribayashi, N
AU  - Kuribayashi N
AU  - et al.
LA  - eng
PT  - Case Reports
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MAX protein, human)
RN  - 0 (Myc associated factor X)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
MH  - Basic-Leucine Zipper Transcription Factors
MH  - Burkitt Lymphoma/genetics/metabolism/*pathology
MH  - Cell Cycle
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 13
MH  - DNA-Binding Proteins/*biosynthesis
MH  - Humans
MH  - Karyotyping
MH  - Male
MH  - Molecular Sequence Data
MH  - Proto-Oncogene Proteins c-myc/*biosynthesis
MH  - *Transcription Factors
MH  - Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1995 Dec;9(12):2093-9.