PMID- 8611030
OWN - NLM
STAT- MEDLINE
DCOM- 19960524
LR  - 20191210
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 326
IP  - 2
DP  - 1996 Feb 15
TI  - Suppression of Alzheimer amyloid precursor protein (APP) expression by exogenous 
      APP mRNA.
PG  - 243-51
AB  - To establish a cell line expressing enhanced levels of beta-amyloid precursor 
      protein (beta-APP), we constructed plasmid DNAs expressing beta-APP-751 mRNA and 
      transfected them into COS-1 cells. Using a modified version of the reverse 
      transcriptase polymerase chain reaction which is RNA sensitive to study the 
      beta-APP iso-RNAs, we have made the unexpected observation that enhanced 
      expression of beta-APP-751 mRNA resulted in a significant reduction of 
      beta-APP-770 and -695 mRNA levels. Suppression of beta-APP-770 and -695 was also 
      observed in cells expressing truncated and chimeric beta-App-751 mRNAs. Similar 
      observations were made in P19 cells expressing a chimeric beta-APP-751 mRNA where 
      endogenous beta-APP-751 mRNA levels also were decreased. Also, suppression of 
      beta-APP-770 and -751 mRNAs was observed in human kidney cells expressing 
      exogenous beta-APP-695 mRNA.
FAU - Ramakrishna, N
AU  - Ramakrishna N
AD  - NYS Institute for Basic Research in Developmental Disabilities, Staten Island 
      10314, USA.
FAU - Smedman, M
AU  - Smedman M
FAU - Gillam, B
AU  - Gillam B
LA  - eng
GR  - AGO4221/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amyloid beta-Protein Precursor/biosynthesis/*genetics
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - DNA/genetics
MH  - Gene Expression
MH  - Humans
MH  - Molecular Sequence Data
MH  - Plasmids/genetics
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics/*metabolism
MH  - Recombinant Fusion Proteins/biosynthesis/genetics
MH  - Transfection
EDAT- 1996/02/15 00:00
MHDA- 1996/02/15 00:01
CRDT- 1996/02/15 00:00
PHST- 1996/02/15 00:00 [pubmed]
PHST- 1996/02/15 00:01 [medline]
PHST- 1996/02/15 00:00 [entrez]
AID - S0003-9861(96)90072-7 [pii]
AID - 10.1006/abbi.1996.0072 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1996 Feb 15;326(2):243-51. doi: 10.1006/abbi.1996.0072.