PMID- 8612324
OWN - NLM
STAT- MEDLINE
DCOM- 19960605
LR  - 20190830
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 89
IP  - 2
DP  - 1995 Nov 21
TI  - The ontogeny of cardiac and neural A1 adenosine receptor expression in rats.
PG  - 202-13
AB  - To provide insights into the sites and mechanisms of adenosine action during 
      fetal life, the ontogeny of A1 adenosine receptor (A1AR) expression was studied 
      in rats. Using in situ hybridization and receptor binding assays, A1AR expression 
      was examined at gestational days (GD) 8, 11, 14, 17, and 21. At GD 8, A1AR mRNA 
      expression was detected in the myocardium but not in other fetal structures. At 
      GD 11, A1AR mRNA was present in the atria, but not in the ventricles or neural 
      structures. At GD 14, A1AR mRNA was present in the atria and the pontine 
      neuroectoderm, the thalamus, and the ventral horn of the spinal cord. At GD 14, 
      A1ARs were first detectable using [3H]DPCPX (8-cyclopentyl-1,3-dipropylxanthine). 
      Functional binding sites were highest in the atria and present at low levels in 
      brain. When GD 17 was reached, patterns of A1AR expression in the brain were 
      similar to those observed in adult animals. Sites of heavy labeling included the 
      spinal cord, medulla, pons, midbrain, thalamus, and the hippocampus. When pre- 
      and postnatal tissue concentrations of A1ARs were compared, cardiac A1AR receptor 
      concentrations were similar. However, postnatal brain A1AR levels were 
      considerably greater than in fetuses. These data identify the atria as a 
      prominent site of fetal A1AR expression and show that the A1AR gene is one of the 
      earliest expressed G protein-coupled receptor genes in the heart. A1AR expression 
      in brain lags behind cardiac expression in early stages of gestation and exceeds 
      cardiac A1AR expression with progressive maturation. A1ARs may therefore 
      influence the heart and brain during critical periods of fetal development.
FAU - Rivkees, S A
AU  - Rivkees SA
AD  - Herman B Wells Center for Pediatric Research, James Whitcomb Riley Hospital, 
      Department of Biochemistry and Molecular Biology, Indiana University School of 
      Medicine, Indianapolis, USA. srivkee/indyvax.iupui.edu
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Purinergic P1)
SB  - IM
MH  - Animals
MH  - Central Nervous System/embryology/metabolism
MH  - Embryonic and Fetal Development/physiology
MH  - Heart/embryology
MH  - Myocardium/*metabolism
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Purinergic P1/*genetics
EDAT- 1995/11/21 00:00
MHDA- 1995/11/21 00:01
CRDT- 1995/11/21 00:00
PHST- 1995/11/21 00:00 [pubmed]
PHST- 1995/11/21 00:01 [medline]
PHST- 1995/11/21 00:00 [entrez]
AID - 0165380695001203 [pii]
AID - 10.1016/0165-3806(95)00120-3 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 1995 Nov 21;89(2):202-13. doi: 
      10.1016/0165-3806(95)00120-3.