PMID- 8612490
OWN - NLM
STAT- MEDLINE
DCOM- 19960605
LR  - 20220311
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 137
IP  - 5
DP  - 1996 May
TI  - Effects of interleukin-1 beta on thyrotropin secretion and thyroid hormone uptake 
      in cultured rat anterior pituitary cells.
PG  - 1591-8
AB  - The effects of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha 
      (TNF alpha) on basal and TRH-induced TSH release, and the effects of IL-1 beta on 
      the uptake of [125I]T3 and [125I]T4 and on nuclear binding of [125I]T3 were 
      examined. Furthermore, the release of other anterior pituitary hormones in the 
      presence of IL-1 beta was measured. Anterior pituitary cells from male Wistar 
      rats were cultured for 3 days in medium containing 10% FCS. Incubation were 
      performed at 37 C in medium with 0.5% BSA for measurement of [125I]T3 uptake and 
      with 0.1% BSA for measurement of [125I]T4 uptake. Exposure to IL-1 beta (1 pM-1 
      nM) or TNF alpha (100 pM) for 2-4 h resulted in a significant decline in TSH 
      release, which was almost 50% (P < 0.05) for 1 nM IL-1 beta and 24% (P < 0.05) 
      for 100 pM TNF alpha. Measurement of other anterior pituitary hormones (FSH, LH, 
      PRL, and ACTH) in the same incubation medium showed that IL-1 beta did not alter 
      their release. When the effects of IL-1 beta (1 pM-1 nM) and TNF alpha (100 pM) 
      on TRH-induced TSH release were measured in short term experiments, the 
      inhibitory effects had disappeared. The addition of 1-100 nM octreotide, a 
      somatostatin analog, resulted in a decrease in TRH-induced TSH release up to 33% 
      of the control value (P < 0.05). Exposure to dexamethasone (1 nM to 1 microM) 
      affected basal and TRH-induced TSH release similar to the effect of IL-1 beta. 
      The 15-min uptake of [125I]T3 and [125I]T4, expressed as femtomoles per pM free 
      hormone, was not affected by the presence of IL-1 beta (1-100 pM). When IL-1 beta 
      (100 pM) was present during 3 days of culture, TSH release was reduced to 88 +/- 
      2% of the control value (P < 0.05). This effect was not associated with an 
      altered [125I]T3 uptake (15 min to 4 h) or with any change in nuclear T3 binding. 
      We conclude that 1) IL-1 beta decreases TSH release by a direct action on the 
      pituitary; 2) this effect is not due to elevated thyroid hormone uptake or 
      increase T3 nuclear occupancy; 3) IL-1 beta does not affect TRH-induced TSH 
      release or the release of other anterior pituitary hormones; and 4) TNF alpha 
      affects basal and TRH-induced TSH release in the same way as IL-1 beta.
FAU - Wassen, F W
AU  - Wassen FW
AD  - Department of Internal Medicine III, Erasmus University Medical School, 
      Rotterdam, The Netherlands.
FAU - Moerings, E P
AU  - Moerings EP
FAU - Van Toor, H
AU  - Van Toor H
FAU - De Vrey, E A
AU  - De Vrey EA
FAU - Hennemann, G
AU  - Hennemann G
FAU - Everts, M E
AU  - Everts ME
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Culture Media)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 5Y5F15120W (Thyrotropin-Releasing Hormone)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9002-71-5 (Thyrotropin)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - Q51BO43MG4 (Thyroxine)
RN  - RWM8CCW8GP (Octreotide)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/drug effects/metabolism
MH  - Cells, Cultured
MH  - Corticotropin-Releasing Hormone/pharmacology
MH  - Culture Media
MH  - Dexamethasone/pharmacology
MH  - Interleukin-1/*pharmacology
MH  - Male
MH  - Octreotide/pharmacology
MH  - Pituitary Gland, Anterior/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Serum Albumin, Bovine
MH  - Thyrotropin/*metabolism
MH  - Thyrotropin-Releasing Hormone/pharmacology
MH  - Thyroxine/*metabolism
MH  - Triiodothyronine/*metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1210/endo.137.5.8612490 [doi]
PST - ppublish
SO  - Endocrinology. 1996 May;137(5):1591-8. doi: 10.1210/endo.137.5.8612490.