PMID- 8612542
OWN - NLM
STAT- MEDLINE
DCOM- 19960605
LR  - 20181130
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 137
IP  - 5
DP  - 1996 May
TI  - The insulin hypoglycemia-induced inhibition of gonadotropin-releasing hormone 
      pulse generator activity in the rhesus monkey: roles of vasopressin and 
      corticotropin-releasing factor.
PG  - 2012-21
AB  - Insulin-induced hypoglycemia (IIH) profoundly inhibits the activity of the 
      hypothalamic GnRH pulse generator. The aim of this study was to determine the 
      role of vasopressin and CRF in this response. Ovariectomized rhesus monkeys with 
      chronically implanted recording electrodes in the mediobasal hypothalamus and 
      with intracerebroventricular (icv) cannulas in the lateral ventricle were placed 
      in primate chairs, and blood samples were taken every 10 min. Pulse generator 
      activity was monitored electrophysiologically by detecting characteristic 
      increases in hypothalamic multi-unit activity (MUA volleys) and by attendant LH 
      pulses measured in peripheral blood. Arginine vasopressin (AVP) infused via the 
      i.c.v. cannula (50 micrograms/60 microliters.h) in eight monkeys failed to 
      decrease pulse generator activity, as measured by the frequency of MUA volleys, 
      but decreased mean serum LH concentrations (P < 0.001) while increasing serum 
      cortisol levels (P < 0.02). Central administration of an AVP antagonist 
      ([deamino-Pen1, O-Me-Tyr2-Arg8] vasopressin) in four monkeys at a rate (180 
      micrograms/60 microliters.h) that had previously been found to block the 
      aforementioned effects of coadministered AVP failed to prevent the IIH-induced 
      inhibition of GnRH pulse generator activity and LH secretion in the same animals. 
      On the other hand, a CRF antagonist, [D-Phe12,Nle21,38,C alpha MeLeu37] rat 
      CRF-(12-41), infused i.c.v. at a rate of 500 micrograms/60 microliters.h in four 
      monkeys, delayed the inhibition of pulse generator frequency in response to IIH. 
      These results suggest that AVP does not mediate the hypoglycemia-induced 
      inhibition of GnRH pulse generator frequency in the rhesus monkey, but that CRF 
      may be involved in this response.
FAU - Chen, M D
AU  - Chen MD
AD  - Department of Integrative Biology, University of Texas Medical School, Houston 
      77225, USA.
FAU - Ordog, T
AU  - Ordog T
FAU - O'Byrne, K T
AU  - O'Byrne KT
FAU - Goldsmith, J R
AU  - Goldsmith JR
FAU - Connaughton, M A
AU  - Connaughton MA
FAU - Knobil, E
AU  - Knobil E
LA  - eng
GR  - HD-08610/HD/NICHD NIH HHS/United States
GR  - HD-17438/HD/NICHD NIH HHS/United States
GR  - T32-HD-07324/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Insulin)
RN  - 11000-17-2 (Vasopressins)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Animals
MH  - Arginine Vasopressin/antagonists & inhibitors/pharmacology
MH  - Cerebral Ventricles/drug effects
MH  - Corticotropin-Releasing Hormone/antagonists & inhibitors/*physiology
MH  - Electrodes
MH  - Electrophysiology
MH  - Female
MH  - Gonadotropin-Releasing Hormone/*physiology
MH  - Hydrocortisone/blood
MH  - Hypoglycemia/*physiopathology
MH  - Hypothalamus, Middle/physiology
MH  - *Insulin
MH  - Luteinizing Hormone/metabolism
MH  - Macaca mulatta
MH  - Ovariectomy
MH  - Rats
MH  - Vasopressins/*physiology
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1210/endo.137.5.8612542 [doi]
PST - ppublish
SO  - Endocrinology. 1996 May;137(5):2012-21. doi: 10.1210/endo.137.5.8612542.